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Fish Glutathione Peroxidase (GSH-PX) ELISA kit

  • 中文名稱:
    魚(yú)谷胱甘肽過(guò)氧化物酶(GSH-PX)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E15930Fh
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    魚(yú)谷胱甘肽過(guò)氧化物酶(GSH-PX)酶聯(lián)免疫試劑盒(CSB-E15930Fh)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的GSH-PX含量。GSH-PX(谷胱甘肽過(guò)氧化物酶)是一種重要的抗氧化酶,能夠清除體內(nèi)的活性氧,保護(hù)細(xì)胞免受氧化損傷。其研究機(jī)制主要涉及GSH-PX在肝臟疾病中的作用,如慢性肝炎、非酒精性脂肪性肝病、酒精性肝病、肝硬化和肝癌等,通過(guò)調(diào)節(jié)氧化應(yīng)激和脂質(zhì)過(guò)氧化反應(yīng),影響疾病的發(fā)生和發(fā)展。試劑盒檢測(cè)范圍為375 μU/mL-6000 μU/mL,適用于魚(yú)類(lèi)生理學(xué)研究、環(huán)境污染物對(duì)機(jī)體氧化損傷評(píng)估、水產(chǎn)飼料抗氧化添加劑效果分析等科研領(lǐng)域,為水產(chǎn)養(yǎng)殖健康管理、生態(tài)毒理學(xué)研究及營(yíng)養(yǎng)調(diào)控提供數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    GSH-PX
  • 種屬:
    Fish
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    375 μU/mL-6000 μU/mL
  • 靈敏度:
    187.5 μU/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 91-99  
    1:200 Average % 103  
    Range % 99-107  
    1:400 Average % 98  
    Range % 93-103  
    1:800 Average % 92  
    Range % 87-97  
  • 回收率:
    The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-104  
    EDTA plasma (n=4) 89 84-94  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μU/ml OD1 OD2 Average    
    6000 0.089 0.084 0.087    
    3000 0.246 0.265 0.256    
    1500 0.551 0.580 0.566    
    750 0.933 0.981 0.957    
    375 1.646 1.569 1.608    
    0 2.362 2.398 2.380    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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