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  4. Fish Heat Shock Protein 70(HSP-70)ELISA Kit

Fish Heat Shock Protein 70(HSP-70)ELISA Kit

  • 中文名稱：
    魚熱休克蛋白70(HSP-70)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E16327Fh
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚熱休克蛋白70(HSP-70)酶聯(lián)免疫試劑盒（CSB-E16327Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HSP70含量。HSP70是重要的熱休克蛋白，在細胞應激反應中起關(guān)鍵作用，廣泛參與蛋白質(zhì)折疊、轉(zhuǎn)運和降解等過程。研究發(fā)現(xiàn)其異常表達與腫瘤、神經(jīng)退行性疾病等多種疾病相關(guān)，抑制或激活HSP70功能正成為潛在治療策略與研究熱點。試劑盒檢測范圍為18.75 pg/mL-300 pg/mL，該產(chǎn)品為科研人員提供可靠工具，可應用于水產(chǎn)養(yǎng)殖健康監(jiān)測、環(huán)境壓力評估、魚類生理機制研究等領(lǐng)域，為水生生物應激反應模型的建立及生態(tài)毒理學實驗提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit
  • 縮寫：
    HSP-70
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 pg/mL-300 pg/mL
  • 靈敏度：
    18.75 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 98  
    Range % 92-103  
    1:4 Average % 82  
    Range % 80-85  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率：
    The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 94 91-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    300 0.153 0.144 0.149    
    150 0.255 0.242 0.249    
    75 0.519 0.533 0.526    
    37.5 1.103 1.134 1.119    
    18.75 2.363 2.270 2.317    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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