Fish Interferon γ,IFN-γ ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

魚γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E12878Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。干擾素-γ（IFNG）是一種重要的細胞因子，主要由活化的T細胞和NK細胞產生，具有調節(jié)免疫狀態(tài)、介導細胞免疫、增強吞噬功能等作用。其研究機制涉及JAK-STAT通路，通過激活下游信號級聯(lián)，介導多種生物反應，如抗病毒、抗腫瘤和免疫調節(jié)。試劑盒檢測范圍為20 pg/mL-4000 pg/mL，適用于水產動物免疫機制研究、感染性疾病模型構建、疫苗效力評估或養(yǎng)殖環(huán)境中魚類免疫狀態(tài)監(jiān)測等場景本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

IFN-gamma，interferon gamma
縮寫：

IFN-
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

20 pg/mL-4000 pg/mL
靈敏度：

10 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	92
	Range %	87-98
1:4	Average %	100
	Range %	97-103
1:8	Average %	53
	Range %	80-86

回收率：

The recovery of fish IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	96	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.