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中文名稱:魚白介素6(IL-6)酶聯(lián)免疫試劑盒
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貨號:CSB-E13258Fh
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規(guī)格:96T/48T
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價格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:魚白介素6(IL-6)酶聯(lián)免疫試劑盒(CSB-E13258Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL-6含量。IL-6是重要的炎癥細胞因子,在免疫調(diào)節(jié)、造血等生理過程中發(fā)揮關(guān)鍵作用。異常升高時與多種疾病如自身免疫病、腫瘤等的發(fā)生發(fā)展密切相關(guān)。其研究機制主要聚焦于阻斷IL-6與其受體結(jié)合,以抑制下游信號通路從而達到治療目的。試劑盒檢測范圍為12.5 pg/mL-200 pg/mL,本試劑盒適用于水產(chǎn)養(yǎng)殖中魚類健康監(jiān)測、病原感染模型建立、免疫增強劑效果評估等科研場景,亦可用于比較醫(yī)學(xué)中脊椎動物免疫系統(tǒng)的進化機制探究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
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縮寫:IL-6
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Uniprot No.:
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種屬:Fish
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:12.5 pg/mL-200 pg/mL
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靈敏度:12.5 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 88-97 1:2 Average % 89 Range % 82-94 1:4 Average % 103 Range % 94-107 1:8 Average % 96 Range % 90-101 -
回收率:
The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 91-104 EDTA plasma (n=4) 91 84-98 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 200 0.128 0.133 0.131 100 0.269 0.258 0.264 50 0.449 0.431 0.440 25 0.851 0.842 0.847 12.5 1.813 1.783 1.798 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Mitigation of benzo(a)pyrene-induced hepatotoxicity and oxidative damage in Nile tilapia (Oreochromis niloticus) using N-acetylcysteine-loaded starch nanoparticles in porous alginate beads through the CYP1A/Nrf2/HO-1 pathway M Abomosallam, A Ateya, AM Shosha, R Refaat,Aquaculture International,2025
- TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
- Interleukin-6 mediates lipopolysaccharide-inhibited irisin secretion in Nile tilapia (Oreochromis niloticus) Y Yan,Fish & shellfish immunology,2023
- Pectin Nanoparticle-Loaded Soft Coral Nephthea sp. Extract as In Situ Gel Enhances Chronic Wound Healing: In Vitro, In Vivo, and In Silico Studies NH Hassan,Pharmaceuticals,2023
- Responses of Earthworms to Acetamiprid NO Erhunmwunse,researchsquare,2023
- Multiple Biomarker Responses in Female Catfish (Clarias Gariepinus) Exposed to Acetaminophen NO Erhunmwunse,Research Square,2021
- Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
- Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Yue Zhang,Fish & shellfish immunology,2020
- Inclusion of condensed tannins in Lateolabrax japonicus diets: Effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance K Peng,Aquaculture Reports,2020
- The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758) I Ghosal,Aquaculture Research,2020
- Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
- Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
- Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
- Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses. Magrone T.et al,Oxid Med Cell Longev.,2016
靶點詳情
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功能:Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
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亞細胞定位:Secreted.
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蛋白家族:IL-6 superfamily
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組織特異性:Expressed in kidney and spleen. Low expression in liver and gills.
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