Fish Interleukin-8,IL-8 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

魚白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒（CSB-E12877Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IL8/CXCL8含量。IL8/CXCL8屬于趨化因子家族，在多種生理和病理過程中發(fā)揮作用，如炎癥反應(yīng)、腫瘤發(fā)展等。研究發(fā)現(xiàn)其能招募免疫細(xì)胞到炎癥部位，參與腫瘤血管生成、細(xì)胞增殖和轉(zhuǎn)移等過程，是炎癥相關(guān)疾病和腫瘤治療研究的潛在靶點(diǎn)。試劑盒檢測范圍為31.25 pg/mL-500 pg/mL，滿足實(shí)驗(yàn)室對魚類疾病模型構(gòu)建、免疫調(diào)節(jié)機(jī)制研究或環(huán)境脅迫響應(yīng)分析的定量檢測需求；支持科研人員高效開展水產(chǎn)動物免疫學(xué)、病理學(xué)等基礎(chǔ)研究，為揭示魚類先天免疫機(jī)制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL8/CXCL8
Uniprot No.：

Q6F4N2
種屬：

Fish
樣本類型：

serum, plasma
檢測范圍：

31.25 pg/mL-500 pg/mL
靈敏度：

31.25 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	92-97
1:2	Average %	95
	Range %	85-100
1:4	Average %	93
	Range %	89-101
1:8	Average %	95
	Range %	86-100

回收率：

The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	85-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.