Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

魚催乳素(PRL/LTH)酶聯(lián)免疫試劑盒（CSB-E12695Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PRL/LTH含量。PRL（泌乳素）和LTH（促乳素，常指PRL）在人體內(nèi)分泌調(diào)節(jié)中起關(guān)鍵作用，與乳腺發(fā)育和泌乳等生理過程相關(guān)。研究聚焦于其在疾病中的機(jī)制，如高泌乳素血癥發(fā)病機(jī)制、藥物對其水平的影響及信號通路探究，為相關(guān)疾病治療提供方向。試劑盒檢測范圍為10 μIU/mL-4000 μIU/mL，廣泛應(yīng)用于魚類繁殖生理學(xué)研究、環(huán)境內(nèi)分泌干擾物評估、水產(chǎn)養(yǎng)殖過程中激素水平監(jiān)測等科研場景，為水生生物內(nèi)分泌機(jī)制探索提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
縮寫：

PRL/LTH
Uniprot No.：

P09319
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

10 μIU/mL-4000 μIU/mL
靈敏度：

10 μIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-100
1:2	Average %	95
	Range %	86-104
1:4	Average %	90
	Range %	85-95
1:8	Average %	96
	Range %	92-108

回收率：

The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102
EDTA plasma (n=4)	95	88-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.