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  4. Fish Superoxide Dismutase(SOD)ELISA kit

Fish Superoxide Dismutase(SOD)ELISA kit

  • 中文名稱：
    魚超氧化物歧化酶(SOD)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15929Fh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚超氧化物歧化酶(SOD)酶聯(lián)免疫試劑盒（CSB-E15929Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SOD含量。SOD即超氧化物歧化酶，是生物體內(nèi)重要抗氧化酶。其背景在于它能清除超氧陰離子自由基，保護(hù)細(xì)胞免受氧化損傷。研究機(jī)制方面，主要是催化超氧陰離子發(fā)生歧化反應(yīng)，生成氧氣和過氧化氫，在抗炎癥、延緩衰老等研究中受關(guān)注。試劑盒檢測(cè)范圍為1.25 ng/mL-20 ng/mL，該產(chǎn)品可為魚類生理學(xué)、環(huán)境毒理學(xué)及水產(chǎn)養(yǎng)殖研究提供可靠工具，例如評(píng)估污染物暴露對(duì)魚類氧化應(yīng)激的影響、監(jiān)測(cè)養(yǎng)殖水體環(huán)境壓力下的生物標(biāo)志物變化，或探究營(yíng)養(yǎng)干預(yù)對(duì)魚類抗氧化系統(tǒng)的調(diào)控機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    sod1 ELISA Kit; cuzn ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
  • 縮寫：
    SOD
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.25 ng/mL-20 ng/mL
  • 靈敏度：
    0.625 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 92-98  
    1:200 Average % 103  
    Range % 100-106  
    1:400 Average % 98  
    Range % 94-102  
    1:800 Average % 92  
    Range % 88-96  
  • 回收率：
    The recovery of fish SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 96-103  
    EDTA plasma (n=4) 89 85-93  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.092 0.099 0.096    
    10 0.143 0.152 0.148    
    5 0.245 0.223 0.234    
    2.5 0.588 0.575 0.582    
    1.25 1.397 1.402 1.400    
    0 2.214 2.352 2.283    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)問答

■ 常見問題解答
Q:

I use the SOD kit(fish) after centrifuge the standard vial at 6000~10000rpm for 30s before opening 2:56 pm
i must pipetting standard vial or not?

A:
Thanks for your inquiry. The standard in this kit is 10 times concentrated and it requires dilutions.For the specific cooperations,pls refer to the "Standard" part stated on page 9 of the manual.I send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    SOD是一種抗氧化酶，它能夠清除自由基，防止氧化損傷。最新的研究表明，SOD不僅在維持細(xì)胞內(nèi)氧化還原平衡中發(fā)揮重要作用，還可能在其他生理和病理過程中發(fā)揮作用。例如，SOD的缺失與許多疾病有關(guān)，包括癌癥、糖尿病和神經(jīng)系統(tǒng)疾病。此外，SOD也被認(rèn)為可能與衰老和長(zhǎng)壽有關(guān)，一些研究表明SOD的增加可能有助于延緩衰老進(jìn)程。
  • 功能：
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 基因功能參考文獻(xiàn)：
    1. fenofibrate almost completely abolished GM-induced reactive oxygen species generation, which seemed to be mediated at least in part by the restoration of the expression of PPARalphadependent antioxidant enzymes, including catalase and superoxide dismutase (SOD)-1. PMID: 28290603
    2. The earliest event in the pathophysiology of amyotrohic lateral sclerosis in the mutant sod1 zebrafish model involves neuronal stress in inhibitory interneurons, resulting from mutant Sod1 expression. PMID: 23281025
    3. A hierarchic gene expression of copper homeostatic genes was demonstrated between atp7a, sp1 and sod1 in zebrafish. PMID: 21167140
    4. depresses cathepsin L activity stimulated by free radicals and prevents otic complications associated with bone erosion PMID: 11771045
    5. This isoform, when expressed in PC-12 cells affects t-butylhydroperoxide-induced apoptosis differentially from its isoenzyme. PMID: 12551919
    6. Copper/zinc superoxide dismutase was cloned from the zebrafish ( Danio rerio). Evidence is presented that SOD protects against paraquat toxicity in fish. PMID: 12876653
    7. Glia maturation factor-null cells ahow a concurrent decrease in CuZnSOD astrocytes. PMID: 15260123

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Cu-Zn superoxide dismutase family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dre:30553

    STRING: 7955.ENSDARP00000064375

    UniGene: Dr.75822