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  4. Fish gonadotropin-releasing hormone,GnRH ELISA Kit

Fish gonadotropin-releasing hormone,GnRH ELISA Kit

  • 中文名稱：
    魚類促性腺激素釋放激素(GnRH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08810f
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4800/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚類促性腺激素釋放激素(GnRH)酶聯(lián)免疫試劑盒（CSB-E08810f）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的GnRH含量。GnRH即促性腺激素釋放激素，是下丘腦分泌的十肽激素。它作用于腺垂體，調(diào)節(jié)促性腺激素合成與釋放，進(jìn)而調(diào)控性腺功能。研究圍繞其分泌調(diào)節(jié)機(jī)制、與受體相互作用、信號(hào)轉(zhuǎn)導(dǎo)通路等展開(kāi)，以探索其在生殖內(nèi)分泌疾病治療中的應(yīng)用。試劑盒檢測(cè)范圍為0.5 ng/mL-20 ng/mL，科研人員可借助本試劑盒開(kāi)展魚類生殖生理機(jī)制研究、水產(chǎn)養(yǎng)殖中人工繁殖技術(shù)優(yōu)化、環(huán)境激素干擾評(píng)估等實(shí)驗(yàn)，例如監(jiān)測(cè)不同發(fā)育階段或環(huán)境脅迫下魚類GnRH的動(dòng)態(tài)變化，分析內(nèi)分泌調(diào)控網(wǎng)絡(luò)對(duì)繁殖行為的影響，為水生生物資源保護(hù)及可持續(xù)養(yǎng)殖提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫：
    GnRH
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.5 ng/mL-20 ng/mL
  • 靈敏度：
    0.5 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 93-104  
    1:2 Average % 94  
    Range % 85-103  
    1:4 Average % 95  
    Range % 83-102  
    1:8 Average % 92  
    Range % 87-101  
  • 回收率：
    The recovery of fish GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-94  
    EDTA plasma (n=4) 94 89-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.134 0.140 0.137    
    10 0.232 0.242 0.237    
    4.6 0.398 0.409 0.404    
    1.5 0.714 0.687 0.701    
    0.5 1.293 1.266 1.280    
    0 2.711 2.740 2.726    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Fish have over a dozen different forms of GnRHs. I would llike to know for which fish GnRH form is this assay designed before placing an order.

A:
Thanks for your inquiry.
This kit is designed against Zebrafish. Pls refer to: http://www.uniprot.org/uniprot/Q5Y835
Q:

If the gene mentioned in the data is indeed gnrh2, the assay cannot be specific for zebrafish or any other fish species. All vertebrate animals, including humans, have gnrh2 gene with mostly unknown function. The GnRH of interest in zebrafish is salmon GnRH or gnrh3 gene. I have to think about it. This kit may not be any better for zebrafish than those for mammalian GnRH because gnrh2 gene encodes for exactly the same functional peptide in all vertebrates.

Do you have data which shows this kit is specific to zebrafish? Could you perhaps send the COA?

A:
Thanks for your inquiry.
GnRH has different isoforms：GnRH，GnRH2 and GnRH3. GnRH-2 is very conservative in different species of fish.
Pls kindly refer to: https://www.nature.com/articles/srep03676.
CSB-E08810f is designed against zebrafish. We tested undiluted serum and plasma samples before and the test value is 0.5--1.5ng/ml.
I will send you COA by email.
Pls let me know if you have any further questions. Thank you.
Q:

Thanks for your response. Based on the literature you provided it seems GnRH2 is not only conserved among fish species but among all vertebrates.
Since I am curious what the difference is between this kit and your kits from other species. Since the antigen is so highly conserved couldn't I use any of your other GnRH kits instead?

A:
Although GnRH2 is highly conserved, different types of animals have different matrix.
Our kit for fish is designed against fish. For different animals, the raw materials used in the kits are different.