Fish metallothionein(MT)ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

魚金屬硫蛋白(MT)酶聯(lián)免疫試劑盒（CSB-EQ027262FI）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的MT含量。試劑盒檢測范圍為25 pg/mL-400 pg/mL，適用于水產(chǎn)養(yǎng)殖研究、水生態(tài)系統(tǒng)重金屬污染監(jiān)測、魚類生理應(yīng)激機(jī)制分析等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MT
種屬：

Fish
樣本類型：

serum, plasma
檢測范圍：

25 pg/mL-400 pg/mL
靈敏度：

12.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	90
	Range %	86-94
1:400	Average %	99
	Range %	94-103
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of fish MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	95	89-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.