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Fish thyroid stimulating hormone(TSH) ELISA Kit

  • 中文名稱:
    魚促甲狀腺素(TSH)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EQ027261FI
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    魚促甲狀腺素(TSH)酶聯(lián)免疫試劑盒(CSB-EQ027261FI)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的TSH含量。TSH即促甲狀腺激素,是腺垂體分泌的促進(jìn)甲狀腺生長(zhǎng)和機(jī)能的激素。其作用機(jī)制主要是調(diào)控甲狀腺細(xì)胞的生長(zhǎng)和甲狀腺血液供應(yīng),促進(jìn)甲狀腺上皮細(xì)胞增生和甲狀腺血液供應(yīng)。在臨床研究中,TSH水平可反映甲狀腺功能狀態(tài),是診斷甲狀腺疾病的重要指標(biāo)。試劑盒檢測(cè)范圍為2.5 μIU/mL-40 μIU/mL,適用于實(shí)驗(yàn)室開展魚類生理研究、內(nèi)分泌干擾物效應(yīng)評(píng)估、水產(chǎn)養(yǎng)殖健康監(jiān)測(cè)等科研場(chǎng)景。該檢測(cè)體系兼容多種樣本類型,為研究魚類甲狀腺功能調(diào)控機(jī)制、環(huán)境污染物對(duì)內(nèi)分泌系統(tǒng)的影響提供可靠工具,特別適用于水生生物毒理學(xué)實(shí)驗(yàn)和基礎(chǔ)生物學(xué)研究中的甲狀腺相關(guān)指標(biāo)分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    tshb ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
  • 縮寫:
    TSH
  • Uniprot No.:
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    2.5 μIU/mL-40 μIU/mL
  • 靈敏度:
    2.5 μIU/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %100
    Range %96-106
    1:2Average %89
    Range %84-94
    1:4Average %94
    Range %90-98
    1:8Average %96
    Range %91-101
  • 回收率:
    The recovery of fish TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-93
    EDTA plasma (n=4)9589-98
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/mlOD1OD2Average
    400.234 0.228 0.231
    200.511 0.502 0.507
    100.888 0.893 0.891
    51.743 1.727 1.735
    2.52.557 2.458 2.508
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Indispensable for the control of thyroid structure and metabolism. May play some role in the biological processes of the immature fishes.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Glycoprotein hormones subunit beta family
  • 組織特異性:
    Pituitary gland. Higher levels seen in immature fishes than the mature fishes.
  • 數(shù)據(jù)庫(kù)鏈接:

    UniGene: Omy.8084