Fish trypsin ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

魚胰蛋白酶(trypsin)酶聯(lián)免疫試劑盒（CSB-EL018811FI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PRSS1含量。PRSS1是一種編碼陽離子胰蛋白酶原的基因，其突變與遺傳性胰腺炎相關(guān)。該基因突變導(dǎo)致胰蛋白酶原激活增加或失活減少，引發(fā)胰腺炎癥。研究顯示，PRSS1突變是慢性胰腺炎的重要遺傳因素，其致病機(jī)制涉及胰蛋白酶的異常激活和胰腺組織的損傷。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，可為水產(chǎn)動(dòng)物消化生理研究、疾病模型構(gòu)建及營養(yǎng)代謝評(píng)估提供重要指標(biāo)；支持實(shí)驗(yàn)室開展魚類消化系統(tǒng)功能研究、水產(chǎn)養(yǎng)殖基礎(chǔ)科研以及環(huán)境因子對(duì)魚類生理影響的毒理學(xué)評(píng)估本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Trypsin-1 ELISA kit; EC 3.4.21.4 ELISA kit; Trypsin I ELISA kit
縮寫：

PRSS1
Uniprot No.：

P35031
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	101
	Range %	95-105
1:40	Average %	91
	Range %	84-95
1:80	Average %	106
	Range %	100-111
1:160	Average %	104
	Range %	97-108

回收率：

The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-100
EDTA plasma (n=4)	102	96-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.