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  4. Goat Heat Shock Protein 70(HSP-70) ELISA KIT

Goat Heat Shock Protein 70(HSP-70) ELISA KIT

  • 中文名稱：
    山羊熱休克蛋白70(HSP-70)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13381G
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊熱休克蛋白70(HSP-70)酶聯(lián)免疫試劑盒（CSB-E13381G）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HSPA2含量。HSPA2，全稱為熱休克蛋白A2，是HSPA（HSP70）分子伴侶家族的一員，對(duì)男性生育能力具有關(guān)鍵作用。存在于多種體細(xì)胞器官中，可能參與調(diào)節(jié)上皮細(xì)胞分化，同時(shí)作為癌癥相關(guān)蛋白，其功能意義尚未達(dá)成共識(shí)。研究顯示，HSPA2在細(xì)胞蛋白質(zhì)質(zhì)量控制中起核心作用，保護(hù)蛋白質(zhì)組，協(xié)助蛋白質(zhì)折疊和運(yùn)輸，激活蛋白水解，并調(diào)節(jié)蛋白質(zhì)復(fù)合物形成。試劑盒檢測(cè)范圍為23.44 ng/mL-1500 ng/mL，在科研領(lǐng)域，可用于探究環(huán)境脅迫、病原感染或代謝異常等因素對(duì)山羊HSP - 70表達(dá)的影響，亦適用于動(dòng)物模型研究、生理機(jī)制解析及免疫調(diào)控相關(guān)實(shí)驗(yàn)，為畜牧科學(xué)、獸醫(yī)學(xué)及基礎(chǔ)生命科學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    HSPA2 ELISA Kit; HSP70-3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Capra hircus (Goat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    23.44 ng/mL-1500 ng/mL
  • 靈敏度：
    5.86 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-99
    1:2Average %92
    Range %85-99
    1:4Average %95
    Range %92-103
    1:8Average %89
    Range %82-98
  • 回收率：
    The recovery of goat HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9589-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    15002.308 2.463 2.386 2.234
    7501.606 1.666 1.636 1.484
    3751.078 1.064 1.071 0.919
    187.50.732 0.721 0.727 0.575
    93.750.428 0.456 0.442 0.290
    46.880.313 0.330 0.322 0.170
    23.440.219 0.235 0.227 0.075
    00.151 0.153 0.152 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.
  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Heat shock protein 70 family