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  4. Goat Interferon γ,IFN-γ ELISA Kit

Goat Interferon γ,IFN-γ ELISA Kit

  • 中文名稱：
    山羊γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12966G
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E12966G）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ，是一種具有多種生物學(xué)功能的細(xì)胞因子。其它在免疫調(diào)節(jié)中至關(guān)重要。研究機(jī)制上，可激活巨噬細(xì)胞、增強(qiáng)細(xì)胞毒性T細(xì)胞的活性，還能調(diào)節(jié)Th1/Th2細(xì)胞平衡，在抗病毒、抗腫瘤及免疫性疾病等研究中受關(guān)注。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，其水平變化常用于評估感染性疾病模型、疫苗效力實(shí)驗(yàn)或免疫調(diào)節(jié)機(jī)制研究；適用于體外定量分析山羊外周血、組織樣本中IFN - γ的動態(tài)表達(dá)，為畜牧領(lǐng)域免疫相關(guān)研究、傳染病防控及疫苗開發(fā)提供可靠的檢測工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Capra hircus (Goat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-96
    1:2Average %103
    Range %99-107
    1:4Average %93
    Range %89-98
    1:8Average %92
    Range %89-94
  • 回收率：
    The recovery of goat IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9793-102
    EDTA plasma (n=4)9188-95
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.833 2.998 2.916 2.818
    2502.257 2.325 2.291 2.193
    1251.742 1.795 1.769 1.671
    62.51.111 1.118 1.115 1.017
    31.20.754 0.784 0.769 0.671
    15.60.377 0.410 0.394 0.296
    7.80.268 0.251 0.260 0.162
    00.101 0.094 0.098
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 組織特異性：
    Released primarily from activated T lymphocytes.
  • 數(shù)據(jù)庫鏈接：

    KEGG: chx:100860815

    UniGene: Chi.38634