Goat Interleukin 10,IL-10 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

山羊白介素10(IL-10)酶聯(lián)免疫試劑盒（CSB-E09809g）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL10含量。IL10，又稱白細(xì)胞介素-10，是一種多效性細(xì)胞因子，主要由單核細(xì)胞和T細(xì)胞產(chǎn)生。它具有抗炎和免疫調(diào)節(jié)功能，通過抑制Th1細(xì)胞因子和抗原呈遞細(xì)胞的功能來發(fā)揮其作用。IL10的研究機(jī)制涉及信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子STAT1、STAT3的激活，以及與其他細(xì)胞因子的相互作用。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于科研領(lǐng)域研究山羊模型中的免疫應(yīng)答機(jī)制、炎癥性疾病進(jìn)展評(píng)估或疫苗開發(fā)中的免疫調(diào)節(jié)分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-10
Uniprot No.：

A7Y3X5
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	82
	Range %	80-85
1:2	Average %	102
	Range %	97-107
1:4	Average %	89
	Range %	84-93
1:8	Average %	107
	Range %	103-111

回收率：

The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	85	80-90

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.