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  4. Goat Interleukin 1β(IL-1β) ELISA Kit

Goat Interleukin 1β(IL-1β) ELISA Kit

  • 中文名稱：
    山羊白介素1β(IL-1β)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E16568G
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E16568G）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B即白介素-1β，是重要炎癥介質(zhì)。它在炎癥反應、免疫調(diào)節(jié)等生理病理過程中起關鍵作用。其研究機制主要圍繞它與受體結合后激活下游信號通路，引發(fā)炎癥級聯(lián)反應，目前針對它的干預研究有望為炎癥相關疾病治療提供新思路。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于動物模型研究、炎癥機制探索、免疫調(diào)控實驗等科研領域，能夠為病原體感染評估、炎癥因子網(wǎng)絡分析、免疫干預效果驗證等基礎研究提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 縮寫：
    IL-1β
  • Uniprot No.：
  • 種屬：
    Capra hircus (Goat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-94
    1:2Average %90
    Range %82-97
    1:4Average %92
    Range %85-100
    1:8Average %95
    Range %90-103
  • 回收率：
    The recovery of goat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-98
    EDTA plasma (n=4)9993-108
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.112 2.052 2.082 1.951
    1001.576 1.654 1.615 1.484
    501.039 0.984 1.012 0.881
    250.587 0.549 0.568 0.437
    12.50.384 0.397 0.391 0.260
    6.250.292 0.269 0.281 0.150
    3.120.208 0.199 0.204 0.073
    00.127 0.134 0.131 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 亞細胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫鏈接：

    UniGene: Chi.15482