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  4. Goat Interleukin 2(IL-2) ELISA Kit

Goat Interleukin 2(IL-2) ELISA Kit

  • 中文名稱：
    山羊白介素2(IL-2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12965G
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E12965G）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的細(xì)胞因子，由T細(xì)胞分泌。其背景在于對(duì)免疫系統(tǒng)有關(guān)鍵調(diào)節(jié)作用。研究機(jī)制上，IL2能與特異性受體結(jié)合，激活T細(xì)胞和NK細(xì)胞等免疫細(xì)胞的增殖與分化，增強(qiáng)機(jī)體免疫功能，在腫瘤免疫治療等方面是研究熱點(diǎn)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于科研領(lǐng)域中對(duì)山羊免疫相關(guān)疾病的機(jī)制探索、炎癥模型分析或疫苗開發(fā)中細(xì)胞免疫應(yīng)答的定量評(píng)估；為獸醫(yī)研究、比較免疫學(xué)及畜牧領(lǐng)域中的免疫調(diào)控研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Capra hircus (Goat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %85-95
    1:2Average %98
    Range %94-102
    1:4Average %102
    Range %94-107
    1:8Average %94
    Range %87-98
  • 回收率：
    The recovery of goat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9795-102
    EDTA plasma (n=4)9388-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.491 2.365 2.428 2.340
    5001.566 1.594 1.580 1.492
    2501.030 1.096 1.063 0.975
    1250.697 0.753 0.725 0.637
    62.50.423 0.443 0.433 0.345
    31.20.256 0.277 0.267 0.179
    15.60.168 0.169 0.169 0.081
    00.087 0.089 0.088
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-2 family
  • 數(shù)據(jù)庫(kù)鏈接：

    UniGene: Chi.38711