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  4. Goat Thyroxine(T4) ELISA Kit

Goat Thyroxine(T4) ELISA Kit

  • 中文名稱：
    山羊甲狀腺素(T4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13384G
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊甲狀腺素(T4)酶聯(lián)免疫試劑盒（CSB-E13384G）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的T4含量。試劑盒檢測范圍為20 ng/mL-320 ng/mL，靈敏度為20 ng/mL。適用于科研領(lǐng)域中對山羊或其他偶蹄目動(dòng)物甲狀腺功能的動(dòng)態(tài)監(jiān)測，例如在營養(yǎng)代謝研究、環(huán)境內(nèi)分泌干擾物評估、激素替代治療模型構(gòu)建中提供定量數(shù)據(jù)支持，亦可應(yīng)用于畜牧學(xué)科研中飼料添加劑對甲狀腺軸影響的藥效學(xué)分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    T4
  • 種屬：
    Capra hircus (Goat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    20 ng/mL-320 ng/mL
  • 靈敏度：
    20 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 86-101    
    1:2 Average % 86    
    Range % 81-98    
    1:4 Average % 95    
    Range % 90-100    
    1:8 Average % 95    
    Range % 90-100    
  • 回收率：
    The recovery of goat T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 92-110    
    EDTA plasma (n=4) 95 90-105    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average 　    
    320 0.163 0.169 0.166 　    
    160 0.275 0.281 0.278 　    
    80 0.493 0.502 0.498 　    
    40 1.015 1.029 1.022 　    
    20 2.049 2.068 2.059 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

相關(guān)問答

■ 常見問題解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13384G,Goat Thyroxine(T4) ELISA Kit *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
The standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.)
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    T4是一種重要的代謝激素，最新研究表明T4與身體免疫系統(tǒng)密切相關(guān)。研究人員正在探索T4對免疫細(xì)胞功能的影響，以及利用T4來治療免疫系統(tǒng)相關(guān)疾病的可能性。