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Goat growth hormone,GH ELISA KIT

  • 中文名稱：
    山羊生長激素(GH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13275G
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    山羊生長激素(GH)酶聯(lián)免疫試劑盒（CSB-E13275G）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 編碼生長激素，其對人體生長發(fā)育至關(guān)重要。研究機制方面，GH1 表達產(chǎn)物生長激素主要通過與受體結(jié)合，激活 JAK-STAT 等信號通路，來調(diào)節(jié)細胞增殖、分化和代謝，影響骨骼、肌肉等組織生長，目前圍繞它的研究有望為生長相關(guān)疾病治療提供思路。試劑盒檢測范圍為6.25 ng/mL-100 ng/mL，本產(chǎn)品適用于科研領(lǐng)域，如動物生理學研究、內(nèi)分泌機制探索、畜牧業(yè)生長調(diào)控優(yōu)化或藥物開發(fā)中GH相關(guān)生物標志物分析，其檢測可為研究山羊生長發(fā)育、代謝疾病模型建立及營養(yǎng)干預效果評估提供重要依據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Capra hircus (Goat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ng/mL-100 ng/mL
  • 靈敏度：
    6.25 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-88  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 95  
    Range % 91-99  
    1:8 Average % 92  
    Range % 88-97  
  • 回收率：
    The recovery of goat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 94 89-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.202 0.200 0.201    
    50 0.396 0.365 0.381    
    25 0.631 0.622 0.627    
    12.5 1.142 1.154 1.148    
    6.25 2.359 2.326 2.343    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.
For example:#CSB-E13275G,Goat growth hormone,GH ELISA KIT *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
The standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶點詳情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能參考文獻：
    1. genetic variation and milk production and composition PMID: 23611522
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: chx:100861230

    UniGene: Chi.38639