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  4. Grouper Vitellogenin,VTG ELISA Kit

Grouper Vitellogenin,VTG ELISA Kit

  • 中文名稱：
    石斑魚卵黃蛋白原(VTG)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14116Fh
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    石斑魚卵黃蛋白原(VTG)酶聯(lián)免疫試劑盒（CSB-E14116Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VTG含量。試劑盒檢測范圍為50 ng/mL-2000 ng/mL，適用于水產(chǎn)養(yǎng)殖中魚類繁殖力評估、環(huán)境污染對生殖系統(tǒng)影響的機(jī)制研究，以及水生態(tài)毒理學(xué)領(lǐng)域的生物監(jiān)測實驗。為海洋生物生殖發(fā)育研究及生態(tài)毒理評估提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    vtg1 ELISA Kit; Vitellogenin ELISA Kit; VTG) [Cleaved into: Lipovitellin I ELISA Kit; LVI); Phosvitin ELISA Kit; PV); Lipovitellin II ELISA Kit; LVII)] ELISA Kit
  • 縮寫：
    VTG
  • Uniprot No.：
  • 種屬：
    Grouper
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    50 ng/mL-2000 ng/mL
  • 靈敏度：
    50 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 84-97  
    1:2 Average % 97  
    Range % 91-102  
    1:4 Average % 95  
    Range % 88-103  
    1:8 Average % 96  
    Range % 91-101  
  • 回收率：
    The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-100  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.174 0.175 0.175    
    1000 0.359 0.378 0.369    
    460 0.567 0.552 0.560    
    150 0.872 0.891 0.882    
    50 1.513 1.476 1.495    
    0 2.389 2.411 2.400    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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