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Guinea pig Immunoglobulin G,IgG ELISA Kit

  • 中文名稱:
    豚鼠免疫球蛋白G(IgG)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E10125Gu
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豚鼠免疫球蛋白G(IgG)酶聯(lián)免疫試劑盒(CSB-E10125Gu)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的IgG含量。IgG是人體免疫系統(tǒng)中的主要抗體,由B細(xì)胞產(chǎn)生,具有抗菌、抗病毒和中和毒素等功能。研究機(jī)制涉及B細(xì)胞分化為漿細(xì)胞,分泌IgG抗體,以及抗體與抗原結(jié)合后的免疫反應(yīng)。近年來(lái),研究發(fā)現(xiàn)腫瘤細(xì)胞也能合成IgG,稱為腫瘤來(lái)源免疫球蛋白(cancer-IgG),其表達(dá)與腫瘤的侵襲和轉(zhuǎn)移密切相關(guān)。試劑盒檢測(cè)范圍為62.5 μg/mL-1000 μg/mL,靈敏度為62.5 μg/mL。該產(chǎn)品適用于科研領(lǐng)域,如感染性疾病模型中免疫應(yīng)答動(dòng)態(tài)監(jiān)測(cè)、疫苗研發(fā)中抗體效價(jià)評(píng)估,或基礎(chǔ)免疫學(xué)研究中豚鼠體液免疫功能分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    IgG
  • 種屬:
    Guinea Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma
  • 檢測(cè)范圍:
    62.5 μg/mL-1000 μg/mL
  • 靈敏度:
    62.5 μg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10 Average % 99  
    Range % 95-103  
    1:20 Average % 94  
    Range % 91-98  
    1:40 Average % 95  
    Range % 90-100  
    1:80 Average % 92  
    Range % 85-96  
  • 回收率:
    The recovery of guinea pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-104  
    EDTA plasma (n=4) 96 84-110  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    1000 0.139 0.141 0.140    
    500 0.300 0.312 0.306    
    250 0.600 0.604 0.602    
    125 0.869 0.872 0.871    
    62.5 1.372 1.395 1.384    
    0 2.916 2.709 2.813    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    IgG 參與感染病毒和細(xì)菌的清除:IgG 抗體可以與病毒或細(xì)菌的表面蛋白結(jié)合,促進(jìn)免疫細(xì)胞清除它們。近期的研究表明,IgG 抗體可以通過(guò)與天然殺傷性 T 細(xì)胞結(jié)合,增強(qiáng)它們清除病原體的能力。
    研究進(jìn)展表明,IgG 在疾病預(yù)防和治療中具有重要作用,并且對(duì)其生物學(xué)和作用機(jī)制的研究仍在不斷深入。