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  4. Guinea pig Interferon γ,IFN-γ ELISA Kit

Guinea pig Interferon γ,IFN-γ ELISA Kit

  • 中文名稱：
    豚鼠γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06763Gu
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E06763Gu）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IFN-γ含量。IFN-γ即γ-干擾素，是具有多種生物學(xué)活性的細(xì)胞因子。背景上，它在免疫調(diào)節(jié)等機(jī)體生理功能中扮演重要角色。研究機(jī)制方面，其可激活巨噬細(xì)胞、增強(qiáng)NK細(xì)胞活性等，在抗感染、抗腫瘤及免疫相關(guān)疾病研究中受廣泛關(guān)注。試劑盒檢測(cè)范圍為18.75 pg/mL-1200 pg/mL，尤其適用于豚鼠模型的感染性疾病機(jī)制探索和疫苗開發(fā)等科研領(lǐng)域；適用于評(píng)估免疫激活狀態(tài)或炎癥反應(yīng)強(qiáng)度；該產(chǎn)品廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)、獸醫(yī)學(xué)研究中宿主免疫應(yīng)答分析、病原體感染模型建立以及生物制劑效果評(píng)價(jià)等非診斷性科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IFN-gamma，interferon gamma
  • 縮寫：
    IFN-γ
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.7 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %81-92
    1:2Average %97
    Range %92-103
    1:4Average %93
    Range %88-108
    1:8Average %101
    Range %96-105
  • 回收率：
    The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9793-102
    EDTA plasma (n=4)9086-95
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    12002.075 2.051 2.063 1.965
    6001.465 1.441 1.453 1.355
    3001.069 1.045 1.057 0.959
    1500.655 0.622 0.639 0.541
    750.400 0.387 0.394 0.296
    37.50.320 0.295 0.308 0.210
    18.750.205 0.223 0.214 0.116
    00.101 0.095 0.098 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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