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  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Guinea pig Interleukin 8,IL-8 ELISA Kit
  • 中文名稱：
    豚鼠白介素8(IL-8)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13096Gp
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠白介素8(IL-8)酶聯(lián)免疫試劑盒（CSB-E13096Gp）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8（C-X-C motif chemokine ligand 8）是一種趨化因子，由巨噬細(xì)胞和其他細(xì)胞產(chǎn)生，具有促進(jìn)血管生成和調(diào)節(jié)炎癥反應(yīng)的功能。其研究機(jī)制涉及對(duì)中性粒細(xì)胞的召集和脫顆粒，以及與多種疾病如癌癥、炎癥和肥胖的關(guān)聯(lián)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，廣泛應(yīng)用于豚鼠模型的感染性疾病機(jī)制研究、免疫調(diào)節(jié)實(shí)驗(yàn)及藥物開(kāi)發(fā)中的炎癥因子動(dòng)態(tài)監(jiān)測(cè)，特別適用于呼吸道感染、皮膚炎癥或腸道疾病等動(dòng)物模型的病理分析，為科研人員提供穩(wěn)定可靠的IL - 8濃度檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant protein 1 ELISA Kit; NAP-1 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-102
    1:2Average %95
    Range %85-100
    1:4Average %95
    Range %88-102
    1:8Average %97
    Range %90-104
  • 回收率：
    The recovery of guinea pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9895-102
    EDTA plasma (n=4)9490-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.253 2.245 2.249 2.134
    10001.631 1.696 1.664 1.549
    5001.111 1.135 1.123 1.008
    2500.717 0.752 0.735 0.620
    1250.434 0.423 0.429 0.314
    62.50.343 0.354 0.349 0.234
    31.250.228 0.241 0.235 0.120
    00.116 0.113 0.115 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：組織勻漿液 肺組織勻漿上清

樣品信息：其他 SPF級(jí)豚鼠

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： 我通過(guò)使用CSB-E13096Gp檢測(cè)了PR8病毒感染的小鼠肺組織勻漿上清，操作簡(jiǎn)單方便易上手，靈敏度非常高，檢測(cè)結(jié)果十分精準(zhǔn)

By 鄧?yán)蠋?/p>

靶點(diǎn)詳情

  • 功能：
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 數(shù)據(jù)庫(kù)鏈接：