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  4. Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

  • 中文名稱：
    豚鼠腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06772Gu
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E06772Gu）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。腫瘤壞死因子（TNF）是一種重要的細(xì)胞因子。其背景上，它在免疫調(diào)節(jié)、炎癥反應(yīng)等生理過程中有關(guān)鍵作用。研究機制方面，TNF可通過與受體結(jié)合，激活多條信號通路，進而調(diào)控細(xì)胞的存活、凋亡及炎癥因子釋放等，在多種疾病發(fā)病機制中扮演重要角色。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，該產(chǎn)品適用于基礎(chǔ)科研中炎癥機制研究、藥物干預(yù)效果評價以及豚鼠模型的免疫相關(guān)疾病實驗，可通過檢測組織勻漿中的 TNF-α 水平分析局部炎癥反應(yīng)，或利用血液樣本評估系統(tǒng)性免疫激活狀態(tài)，其表達(dá)水平的變化常用于評估感染性疾病、自身免疫反應(yīng)或腫瘤研究中的免疫狀態(tài)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %90-100
    1:2Average %94
    Range %89-98
    1:4Average %92
    Range %87-96
    1:8Average %93
    Range %89-100
  • 回收率：
    The recovery of guinea pig TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-97
    EDTA plasma (n=4)9792-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.350 2.376 2.363 2.200
    1001.884 1.902 1.893 1.730
    501.368 1.379 1.374 1.211
    250.961 0.973 0.967 0.804
    12.50.664 0.681 0.673 0.510
    6.250.475 0.486 0.481 0.318
    3.120.305 0.309 0.307 0.144
    00.162 0.164 0.163 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：組織勻漿液 肺組織勻漿上清

樣品信息：其他 SPF級豚鼠

稀釋比：沒有稀釋

產(chǎn)品評價： 我通過使用CSB-E06772Gu檢測了PR8病毒感染的小鼠肺組織勻漿上清，操作簡單方便易上手，靈敏度非常高，檢測結(jié)果十分精準(zhǔn)

By 鄧?yán)蠋?/p>

靶點詳情

  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數(shù)據(jù)庫鏈接：