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  4. Guinea pig insulin (INS) ELISA kit

Guinea pig insulin (INS) ELISA kit

  • 中文名稱(chēng)：
    豚鼠胰島素(INS)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011742GU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠胰島素(INS)酶聯(lián)免疫試劑盒（CSB-EL011742GU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的INS含量。INS 即胰島素基因，其編碼的胰島素是調(diào)節(jié)血糖的關(guān)鍵激素。在研究機(jī)制上，主要聚焦于其表達(dá)調(diào)控、胰島素信號(hào)通路等。深入研究有助于理解糖尿病等代謝疾病發(fā)病機(jī)制，也為開(kāi)發(fā)相關(guān)治療藥物，如胰島素類(lèi)似物等提供理論基礎(chǔ)。試劑盒檢測(cè)范圍為12 μIU/mL-210 μIU/mL，適用于豚鼠模型中胰島素相關(guān)科研分析；在糖尿病機(jī)制研究、代謝性疾病模型構(gòu)建及藥物干預(yù)效果評(píng)價(jià)等領(lǐng)域具有重要應(yīng)用價(jià)值；可為胰腺功能研究、胰島素分泌調(diào)控實(shí)驗(yàn)提供精準(zhǔn)數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    12 μIU/mL-210 μIU/mL
  • 靈敏度：
    7.5 μIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-97  
    1:2 Average % 96  
    Range % 90-106  
    1:4 Average % 95  
    Range % 86-104  
    1:8 Average % 90  
    Range % 87-94  
  • 回收率：
    The recovery of guinea pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-101  
    EDTA plasma (n=4) 95 89-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    12 0.049 0.051 0.050    
    24 0.165 0.160 0.163    
    48 0.377 0.374 0.376    
    120 0.853 0.849 0.851    
    210 1.686 1.691 1.689    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫(kù)鏈接：