Guinea pig interleukin 10,IL-10 ELISA Kit

產(chǎn)品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豚鼠白介素10(IL-10)酶聯(lián)免疫試劑盒（CSB-E12915Gp）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL10含量。IL10是重要的細胞因子，其背景在于它具有免疫調(diào)節(jié)作用，能抑制多種促炎因子釋放。研究機制方面，主要通過與其受體結(jié)合，激活下游信號通路，調(diào)控免疫細胞活性和功能，從而在炎癥、自身免疫病等疾病研究中備受關(guān)注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研場景下豚鼠感染性疾病模型、過敏反應(yīng)研究、免疫調(diào)節(jié)劑開發(fā)等領(lǐng)域的IL-10表達水平分析，尤其適合評估實驗藥物或生物制劑對免疫通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
縮寫：

IL10
Uniprot No.：

Q9Z1Y5
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	96
	Range %	91-103
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	91-100

回收率：

The recovery of guinea pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-101
EDTA plasma (n=4)	97	91-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
亞細胞定位：

Secreted.
蛋白家族：

IL-10 family