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  4. Guinea pig interleukin 4,IL-4 ELISA Kit

Guinea pig interleukin 4,IL-4 ELISA Kit

  • 中文名稱：
    豚鼠白介素4(IL-4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E10126Gu
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豚鼠白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E10126Gu）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-4含量。IL-4是一種多效細胞因子，參與免疫應答的調(diào)節(jié)。主要由肥大細胞、T細胞等分泌，通過IL-4 RA受體招募γc鏈，形成I型信號復合物，介導細胞生長、抗凋亡、基因激活和分化。此外，IL-4還參與調(diào)節(jié)T細胞分化、免疫球蛋白類轉(zhuǎn)換等，在免疫和炎癥過程中發(fā)揮重要作用。試劑盒檢測范圍為3.12 pg/mL-200pg/mL，適用于豚鼠模型的免疫機制研究，特別是在過敏性疾病、寄生蟲感染、哮喘模型等Th2型免疫反應相關(guān)科研領(lǐng)域，為評估實驗動物體內(nèi)IL - 4動態(tài)變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-4
  • Uniprot No.：
  • 種屬：
    Guinea Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %93-105
    1:2Average %96
    Range %92-102
    1:4Average %103
    Range %97-107
    1:8Average %84
    Range %80-88
  • 回收率：
    The recovery of guinea pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9590-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.375 2.294 2.335 2.160
    1001.903 1.802 1.853 1.678
    501.355 1.376 1.366 1.191
    250.903 0.914 0.909 0.734
    12.50.650 0.641 0.646 0.471
    6.250.423 0.410 0.417 0.242
    3.120.317 0.312 0.315 0.140
    00.176 0.173 0.175 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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