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  4. Hamster Interleukin 1β,IL-1β ELISA Kit

Hamster Interleukin 1β,IL-1β ELISA Kit

  • 中文名稱：
    倉鼠白介素1β (IL-1β)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14259Ha
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    倉鼠白介素1β (IL-1β)酶聯(lián)免疫試劑盒（CSB-E14259Ha）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL1B含量。IL1B 即白介素-1β，是一種促炎細(xì)胞因子。它在炎癥反應(yīng)、免疫調(diào)節(jié)等生理和病理過程中扮演重要角色。研究表明，IL1B 可激活下游信號(hào)通路，促進(jìn)炎癥因子釋放，與多種炎癥性疾病、自身免疫性疾病的發(fā)生發(fā)展密切相關(guān)，是潛在的治療靶點(diǎn)。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于體外炎癥模型構(gòu)建、免疫調(diào)控機(jī)制研究以及生物制劑或天然產(chǎn)物的抗炎效果評價(jià)等科研場景，為探索IL - 1β在倉鼠疾病模型中的生物學(xué)功能提供可靠工具。其IL - 1β動(dòng)態(tài)變化常用于評估感染性疾病模型、自身免疫機(jī)制及炎癥相關(guān)藥物干預(yù)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-1β
  • Uniprot No.：
  • 種屬：
    Mesocricetus auratus (Golden hamster)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.12 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %105
    Range %100-112
    1:4Average %89
    Range %84-93
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of hamster IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-99
    EDTA plasma (n=4)8480-88
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    8002.578 2.627 2.603 2.460
    4002.009 2.106 2.058 1.915
    2001.227 1.267 1.247 1.104
    1000.571 0.535 0.553 0.410
    500.308 0.329 0.319 0.176
    250.231 0.219 0.225 0.082
    12.50.204 0.215 0.210 0.067
    00.145 0.140 0.143
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)