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  4. Hamster Interleukin-4(IL4) ELISA kit

Hamster Interleukin-4(IL4) ELISA kit

  • 中文名稱：
    倉鼠白介素4(IL4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011659HA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    倉鼠白介素4(IL4)酶聯(lián)免疫試劑盒（CSB-EL011659HA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL4含量。IL4是重要的免疫細胞因子靶點。其背景在于它在免疫調(diào)節(jié)中扮演關鍵角色，參與Th2細胞分化等過程。研究機制上，主要聚焦其與受體結合后的信號傳導通路，以及在過敏、哮喘等疾病發(fā)生發(fā)展中的作用，旨在開發(fā)相關靶向療法。試劑盒檢測范圍為9.37 pg/mL-600 pg/mL，適用于倉鼠免疫機制研究、炎癥性疾病模型評估、疫苗或藥物開發(fā)中的免疫應答分析等科研場景，為探究Th2型免疫反應、過敏性疾病機制或感染病理過程提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mesocricetus auratus (Golden hamster)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    9.37 pg/mL-600 pg/mL
  • 靈敏度：
    2.34 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 90-101
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 100
    Range % 98-104
    1:8 Average % 94
    Range % 87-97
  • 回收率：
    The recovery of hamster IL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 93-99
    EDTA plasma (n=4) 91 87-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    600 1.820 1.864 1.842 1.628
    300 1.357 1.289 1.323 1.109
    150 0.930 0.963 0.947 0.733
    75 0.706 0.739 0.723 0.509
    37.5 0.503 0.524 0.514 0.300
    18.75 0.392 0.384 0.388 0.174
    9.37 0.304 0.288 0.296 0.082
    0 0.216 0.212 0.214 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family