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  4. Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

  • 中文名稱：
    倉鼠假定磷脂酶B樣蛋白2(PLBD2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018125HA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    倉鼠假定磷脂酶B樣蛋白2(PLBD2)酶聯(lián)免疫試劑盒（CSB-EL018125HA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PLBD2含量。倉鼠PLBD2是一種假定的磷脂酶，與IGF2R相互作用，可能參與生長和信號傳導(dǎo)。其磷脂酶活性尚不明確，但研究可能揭示其在細(xì)胞穩(wěn)態(tài)和調(diào)節(jié)機(jī)制中的作用。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，科研人員可將其應(yīng)用于基礎(chǔ)研究領(lǐng)域，例如探索PLBD2在脂質(zhì)代謝途徑中的功能機(jī)制、評估特定疾病模型中該蛋白的表達(dá)動(dòng)態(tài)變化，或用于細(xì)胞信號轉(zhuǎn)導(dǎo)相關(guān)實(shí)驗(yàn)的分子標(biāo)記物檢測。適用于分子生物學(xué)實(shí)驗(yàn)室常規(guī)蛋白定量研究需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    P76, LAMA-like protein 2|PLB homolog 2|lamina ancestor homolog 2|mannose-6-phosphate protein associated protein p76|phospholipase B-like 2 32 kDa form|phospholipase B-like 2 45 kDa form
  • 縮寫：
    PLBD2
  • 種屬：
    Mesocricetus auratus (Golden hamster)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.12 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of hamster PLBD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-105
    1:2Average %92
    Range %86-96
    1:4Average %95
    Range %90-100
    1:8Average %95
    Range %91-100
  • 回收率：
    The recovery of hamster PLBD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-94
    EDTA plasma (n=4)9689-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.349 2.367 2.358 2.227
    4001.975 1.924 1.950 1.819
    2001.408 1.466 1.437 1.306
    1000.912 0.857 0.885 0.754
    500.555 0.521 0.538 0.407
    250.363 0.374 0.369 0.238
    12.50.248 0.259 0.254 0.123
    00.133 0.129 0.131 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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