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  4. Horse Erythropoietin(EPO) ELISA kit

Horse Erythropoietin(EPO) ELISA kit

  • 中文名稱：
    馬紅細(xì)胞生成素(EPO)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL007743HO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    馬紅細(xì)胞生成素(EPO)酶聯(lián)免疫試劑盒（CSB-EL007743HO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的EPO含量。EPO（促紅細(xì)胞生成素）是一種糖蛋白激素，主要在腎臟產(chǎn)生，負(fù)責(zé)調(diào)節(jié)紅細(xì)胞生成。其研究機(jī)制涉及EPO與受體結(jié)合，通過(guò)JAK2-STAT5通路促進(jìn)紅細(xì)胞生成，并在類風(fēng)濕關(guān)節(jié)炎等疾病中發(fā)揮重要作用。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于科研領(lǐng)域探索EPO在生理或病理?xiàng)l件下的表達(dá)規(guī)律，例如研究貧血相關(guān)疾病機(jī)制、慢性腎病引起的紅細(xì)胞生成異常、腫瘤微環(huán)境對(duì)造血功能的干擾，或評(píng)估藥物及生物活性分子對(duì)EPO信號(hào)通路的影響?？蔀轶w外實(shí)驗(yàn)提供精準(zhǔn)的定量數(shù)據(jù)支持，助力于血液學(xué)、腫瘤生物學(xué)及代謝調(diào)控等領(lǐng)域的分子機(jī)制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    EPOErythropoietin ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Equus caballus (Horse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of horse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 82-91
    1:2 Average % 84
    Range % 80-88
    1:4 Average % 95
    Range % 90-100
    1:8 Average % 98
    Range % 94-102
  • 回收率：
    The recovery of horse EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 85-97
    EDTA plasma (n=4) 106 101-113
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.657 2.546 2.602 2.498
    5 2.076 1.993 2.035 1.931
    2.5 1.400 1.380 1.390 1.286
    1.25 0.761 0.803 0.782 0.678
    0.625 0.458 0.434 0.446 0.342
    0.312 0.295 0.315 0.305 0.201
    0.156 0.219 0.221 0.220 0.116
    0 0.101 0.107 0.104 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 數(shù)據(jù)庫(kù)鏈接：