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  4. Horse Tumor necrosis factor α,TNF-α ELISA Kit

Horse Tumor necrosis factor α,TNF-α ELISA Kit

  • 中文名稱：
    馬腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14031Hs
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    馬腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E14031Hs）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNF含量。TNF（腫瘤壞死因子）是一種多功能促炎細(xì)胞因子，主要由巨噬細(xì)胞分泌，與腫瘤壞死因子受體結(jié)合發(fā)揮功能。其在細(xì)胞凋亡、炎癥和免疫反應(yīng)中起重要作用，與多種疾病如腫瘤、炎癥和自身免疫性疾病相關(guān)。研究TNF機(jī)制有助于開(kāi)發(fā)針對(duì)相關(guān)疾病的治療策略。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于科研場(chǎng)景下對(duì)馬屬動(dòng)物炎癥相關(guān)模型的機(jī)制研究、藥物干預(yù)效果評(píng)價(jià)或基礎(chǔ)免疫學(xué)分析，例如通過(guò)檢測(cè)血清或組織中的TNF-α濃度動(dòng)態(tài)，揭示炎癥相關(guān)疾病的分子調(diào)控網(wǎng)絡(luò)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Equus caballus (Horse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of horse TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %86
    Range %80-97
    1:2Average %92
    Range %84-99
    1:4Average %95
    Range %92-103
    1:8Average %92
    Range %86-98
  • 回收率：
    The recovery of horse TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9283-98
    EDTA plasma (n=4)9491-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.601 2.548 2.575 2.409
    5002.248 2.211 2.230 2.064
    2501.631 1.616 1.624 1.458
    1250.905 0.925 0.915 0.749
    62.50.475 0.483 0.479 0.313
    31.20.312 0.321 0.317 0.151
    15.60.255 0.268 0.262 0.096
    00.164 0.167 0.166
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 基因功能參考文獻(xiàn)：
    1. The results obtained showed that IL-6 expression in adipose tissue biopsies derived from animals with equine metabolic syndrome was enhanced while TNF-alpha levels of both groups were comparable. PMID: 25269712
    2. Data indicate that tumor necrosis factor-alpha production is closely related to ovarian steroid actions. PMID: 24642167
    3. These results indicate that in equine corpus luteum, cytokines TNF, IFNG and FASL regulate nitric oxide activity, via eNOS expression modulation. PMID: 23941776
    4. These data show the presence of cytokines TNF and IFNG, and their receptors, in the equine corpus luteum and indicate their potential involvement in regulation of luteal function. PMID: 22186103
    5. These results suggest that basal oxidative stress markers, circulating cytokines and anti-inflammatory neuroendocrine hormones appear to correlate with endurance performance in horses. PMID: 21058993
    6. The contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of IL-6, IL-10 and TNF-alpha, in horses with recurrent airway obstruction during exacerbation and in remission is reported. PMID: 18294392
    7. The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID: 18584861

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族：
    Tumor necrosis factor family
  • 數(shù)據(jù)庫(kù)鏈接：