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Horse growth hormone,GH ELISA Kit

  • 中文名稱：
    馬生長激素(GH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11242Eq
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4800/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    馬生長激素(GH)酶聯(lián)免疫試劑盒（CSB-E11242Eq）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 為生長激素 1 基因，其表達產(chǎn)物生長激素與人體生長發(fā)育密切相關(guān)。研究機制方面，它主要通過與受體結(jié)合，激活多種信號通路，促進細胞增殖、分化和蛋白質(zhì)合成。異常的 GH1 表達或功能可能引發(fā)如巨人癥、侏儒癥等生長異常疾病。試劑盒檢測范圍為25 ng/mL-400 ng/mL，適用于科研領(lǐng)域探究馬匹生長調(diào)控、營養(yǎng)代謝、繁殖性能及疾病模型中的GH動態(tài)變化。適用于實驗室對馬科動物生理病理機制研究、激素相關(guān)藥物開發(fā)評估以及畜牧領(lǐng)域GH水平監(jiān)測等科研需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Equus caballus (Horse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    25 ng/mL-400 ng/mL
  • 靈敏度：
    25 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of horse GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %87
    Range %84-90
    1:4Average %96
    Range %90-102
    1:8Average %90
    Range %85-96
  • 回收率：
    The recovery of horse GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-98
    EDTA plasma (n=4)9691-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    4000.202 0.204 0.203
    2000.356 0.346 0.351
    1000.597 0.582 0.590
    501.102 1.104 1.103
    252.319 2.354 2.337
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能參考文獻：
    1. Plasma levels of growth hormone (as well as glucose, insulin, and leptin) are highly correlated with the duration of winter anovulatory phase. PMID: 21958120
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：