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  4. Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

Human α-melanocyte stimulating hormone(α-MSH) ELISA Kit

  • 中文名稱：
    人α-黑色素細(xì)胞刺激素(α-MSH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09658h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人α-黑色素細(xì)胞刺激素(α-MSH)酶聯(lián)免疫試劑盒（CSB-E09658h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的α-MSH含量。α - MSH即α - 促黑素細(xì)胞激素，是一種內(nèi)源性神經(jīng)肽。它在調(diào)節(jié)皮膚色素沉著、能量代謝、炎癥反應(yīng)等方面發(fā)揮重要作用。研究機(jī)制主要涉及與黑素皮質(zhì)素受體結(jié)合，激活下游信號(hào)通路，進(jìn)而對(duì)多種生理功能產(chǎn)生影響。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品廣泛應(yīng)用于基礎(chǔ)科研領(lǐng)域，如探究α-MSH在代謝綜合征動(dòng)物模型中的表達(dá)特征、評(píng)估體外細(xì)胞刺激實(shí)驗(yàn)的激素分泌水平，或解析皮膚黑色素生成調(diào)控機(jī)制等研究場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    alpha-melanocyte stimulating hormone, alpha-MSH
  • 縮寫：
    α-MSH
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Biochemicals
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %92-104
    1:2Average %95
    Range %91-104
    1:4Average %89
    Range %85-93
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of human α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9289-95
    EDTA plasma (n=4)9489-97
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.081 2.181 2.131 2.000
    51.256 1.198 1.227 1.096
    2.50.655 0.635 0.645 0.514
    1.250.406 0.416 0.411 0.280
    0.6250.260 0.270 0.265 0.134
    0.3120.220 0.219 0.220 0.089
    0.1560.187 0.182 0.185 0.054
    00.132 0.130 0.131 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：細(xì)胞培養(yǎng)上清液

樣品信息：人

稀釋比：沒有稀釋

產(chǎn)品評(píng)價(jià)： 我用的是人源α-MSH試劑盒。本次實(shí)驗(yàn)?zāi)康模禾骄縐VB對(duì)角質(zhì)細(xì)胞分泌α-MSH的影響及多肽樣品的作用。實(shí)驗(yàn)樣品：UVB照射后，細(xì)胞培養(yǎng)箱中孵育24h，收取細(xì)胞分泌上清液。實(shí)驗(yàn)組OD值是0.15-0.31。以上為近期所用后的反饋，請(qǐng)查收！

By 雷老師