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  4. Human 14-3-3 protein zeta/delta(YWHAZ) ELISA kit

Human 14-3-3 protein zeta/delta(YWHAZ) ELISA kit

  • 中文名稱：
    人14-3-3 ζ/δ蛋白(YWHAZ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL026293HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人14-3-3 ζ/δ蛋白(YWHAZ)酶聯(lián)免疫試劑盒（CSB-EL026293HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的YWHAZ含量。YWHAZ 即 14-3-3ζ 蛋白，廣泛參與細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)。其背景在于它是 14-3-3 蛋白家族成員，與腫瘤發(fā)生、發(fā)展關(guān)系密切。研究機(jī)制上，它可結(jié)合多種磷酸化蛋白，調(diào)控細(xì)胞增殖、凋亡、遷移等過程，有望成為腫瘤治療新靶點(diǎn)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，為研究YWHAZ在疾病模型中的功能調(diào)控、藥物干預(yù)效果評(píng)估或生物標(biāo)志物篩選等科研場景提供精準(zhǔn)定量工具，實(shí)驗(yàn)數(shù)據(jù)可輔助揭示目標(biāo)蛋白在特定生理或病理?xiàng)l件下的動(dòng)態(tài)變化規(guī)律。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    14 3 3 delta ELISA Kit; 14 3 3 protein zeta/delta ELISA Kit; 14 3 3 protein/cytosolic phospholipase A2 ELISA Kit; 14 3 3 zeta ELISA Kit; 14-3-3 protein zeta/delta ELISA Kit; 1433Z_HUMAN ELISA Kit; Epididymis luminal protein 4 ELISA Kit; Epididymis secretory protein Li 3 ELISA Kit; HEL S 3 ELISA Kit; HEL4 ELISA Kit; KCIP-1 ELISA Kit; KCIP1 ELISA Kit; MGC111427 ELISA Kit; MGC126532 ELISA Kit; MGC138156 ELISA Kit; Phospholipase A2 ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; delta polypeptide ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; zeta polypeptide ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein; zeta polypeptide ELISA Kit; YWHAD ELISA Kit; YWHAZ ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-99  
    1:2 Average % 89  
    Range % 85-95  
    1:4 Average % 97  
    Range % 90-105  
    1:8 Average % 95  
    Range % 87-100  
  • 回收率：
    The recovery of human YWHAZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-103  
    EDTA plasma (n=4) 92 88-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.454 2.488 2.471 2.334  
    20 2.049 2.019 2.034 1.897  
    10 1.441 1.394 1.418 1.281  
    5 0.908 0.868 0.888 0.751  
    2.5 0.595 0.557 0.576 0.439  
    1.25 0.352 0.356 0.354 0.217  
    0.625 0.265 0.255 0.260 0.123  
    0 0.136 0.137 0.137 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.
  • 基因功能參考文獻(xiàn)：
    1. Amount of 14-3-3 proteins is decreased in pineal gland, blood platelets and ileum of patients with ASD. PMID: 28522826
    2. These results imply that disorder in the N-terminal helices of 14-3-3 zeta is a consequence of the dimer-monomer dynamics and may play a role in conferring chaperone function to 14-3-3 zeta protein. PMID: 29109150
    3. Knockdown of YWHAZ inhibited cell cycle progression, migration, and the expression of stem cell markers and tumorigenicity was suppressed in tumor-bearing BALB/c nude mice. The expression of YWHAZ was directly down-regulated by miR-30e in resistant ovarian cancer cells. PMID: 30134224
    4. our data suggest miR-204 and 14-3-3zeta as potential therapeutic targets in osteosarcoma PMID: 29441884
    5. evidence is lacking to conclude that 14-3-3zeta is a useful marker of tamoxifen resistance. PMID: 28643021
    6. TRIM21 positively regulated osteosarcoma cell proliferation. Overexpression of TRIM21 enhanced osteosarcoma cell tolerance toward various stresses. YWHAZ protein was identified as a novel interacting partner of TRIM21 and its expression levels were negatively regulated by TRIM21. PMID: 29673441
    7. several disordered regions of PI4KB become protected from proteolytical degradation upon 14-3-3 binding. PMID: 28864297
    8. Ectopic expression of miR-451 could inhibit the cell migration and invasion, promoted apoptosis, induced cell-cycle arrest Furthermore, tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein zeta (YWHAZ) was identified as a direct target of miR-451 PMID: 28981108
    9. Serum autoantibodies to YWHAZ are produced at substantially greater levels in gastric cancer patients as compared to controls. PMID: 28944820
    10. Dimerization of 14-3-3 zeta (14-3-3zeta) dimer was disrupted by a double mutant (L12E, M78K). PMID: 29203375
    11. Results identified YWHAZ as the direct target of miR-613 in hepatocellular carcinoma (HCC). Its overexpression reverses the tumor suppressing role of miR-613 in HCC cells. PMID: 29551505
    12. 14-3-3zetaoverexpression might be a potential prognostic biomarker for ovarian cancer. PMID: 29214776
    13. In AML patients, low level of miR-451 is negatively correlated with high levels of c-Myc and YWHAZ, while c-Myc level is positively related to YWHAZ expression. These results suggested that c-Myc dash, verticalmiR-451 dash, verticalYWHAZ/AKT cascade might play a crucial role during leukemogenesis, and reintroduction of miR-451 could be as a potential strategy for AML therapy. PMID: 27764807
    14. miR-22 exhibits tumor-suppressive effects in hepatocellular carcinoma cells by regulating YWHAZ/AKT/FOXO3a signaling. PMID: 27811373
    15. our data demonstrate that overexpression of 14-3-3zeta in early stage pre-cancerous breast epithelial cells may trigger an elevated glycolysis and transcriptionally up-regulating LDHA, thereby contributes to human breast cancer initiation. PMID: 27150057
    16. 14-3-3zeta can bind to the FOXO3a transcription factor to promote the export of the complex to the cytoplasm, leading to enhanced proliferation and migration of tongue cancer cells. PMID: 27080223
    17. Structure of the complex of phosphorylated liver kinase B1 and 14-3-3zeta has been reported. PMID: 28368277
    18. These results suggest that the hypoxia/14-3-3zeta/HIF-1alpha pathway plays an important role in portal vein tumor thrombus formation and hepatocellular carcinoma metastasis PMID: 26910835
    19. 14-3-3zeta recruited YAP and p-LATS to form a complex under high cells density status and 14-3-3zeta other than YAP or phospho-LATS was the key regulatory molecule of this complex. PMID: 27334574
    20. This study shows that human procaspase-2 interaction with 14-3-3 zeta is governed by phosphorylation at both S139 and S164. PMID: 28943433
    21. The results highlight a new role of TSC2 in protecting glioblastoma against photodynamic therapy-induced cell death, and TSC2 and YWHAZ as new RIP3 partners. PMID: 27984090
    22. These results suggest that 14-3-3-zeta is involved in the TLR3-TICAM-1 pathway in promoting multimerization of TICAM-1 for the formation of a TICAM-1 signalosome. PMID: 27058640
    23. The data indicate that microtubule-bound tau is resistant to 14-3-3zeta-induced tau aggregation and suggest that tau phosphorylation promotes tau aggregation in the brain by detaching tau from microtubules and thus making it accessible to 14-3-3zeta. PMID: 27548710
    24. Structural interface between LRRK2 and 14-3-3 delta protein has been presented. PMID: 28202711
    25. 14-3-3zeta-mediated invasion of cancer cells was found to upregulate Snail through the activation of atypical protein kinase C (aPKC). PMID: 27554601
    26. results have identified a novel mechanism by which 14-3-3sigma maintains the epithelial phenotype by inhibiting Epithelial to Mesenchymal Transition and suggest that this property of 14-3-3sigma might contribute to its function as a tumor suppressor gene. PMID: 27261462
    27. 14-3-3zeta regulates HIF-1alpha production in hepatocellular carcinoma cells by directly binding to HIF-1alpha and via PI3K/Akt/NF-small ka, CyrillicB signal transduction pathway. PMID: 26884855
    28. Results indicate that HuR induces 14-3-3zeta translation via interaction with its 3' UTR and that 14-3-3zeta is necessary for stimulation of intestinal epithelial cell migration after wounding. PMID: 27401462
    29. this study suggests that the down-regulation of 14-3-3 zeta leads to the inhibition of TGFb1- induced contraction by decreasing the expression of total RhoA in TM cells. PMID: 26906158
    30. loss of expression or even the down-regulation of c-abl, but not WYHAZ, is a fundamental event that leads to genesis and progression of tumors PMID: 26429164
    31. This study provides the molecular basis for C-Raf C-terminal-derived phosphopeptide interaction with 14-3-3zeta protein and gives structural insights responsible for phosphorylation-mediated protein binding. PMID: 26295714
    32. 14-3-3z may play an important role in signaling pathway in breast cancer. Also, a high 14-3-3z expression could positively regulate growth factor receptors and protein kinase pathways PMID: 25861752
    33. Studies show that 14-3-3zeta is overexpressed in oral squamous cell carcinoma and provide evidence that may regulate tumor inflammation and immune response through Stat3 signaling. PMID: 25556369
    34. Activation of PCTAIRE-1 is mediated through interaction with the phosphorylated form of cyclin Y in complex with 14-3-3. PMID: 26205494
    35. C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer. PMID: 25971962
    36. Our findings indicate that YWHAZ could serve as a promising prognostic biomarker in localized PCa to predict poor prognosis PMID: 25156059
    37. studyconfirmed the interaction of Ser9-phosphorylated GSK3beta with 14-3-3zeta; Ser9-phosphorylation of GSK3beta promoted by 14-3-3zeta is critical for the activation of NF-kappaB pathway PMID: 25138042
    38. A detailed analysis of the interaction between singly or doubly phosphorylated human tyrosine hydroxylase isoform 1(1-50) peptides and 14-3-3zeta PMID: 25418103
    39. BIS targeting induces cellular senescence through the regulation of 14-3-3 zeta/STAT3/SKP2/p27 in glioblastoma cells. PMID: 25412315
    40. Aberrant upregulation of 14-3-3sigma and EZH2 expression serves as an inferior prognostic biomarker for hepatocellular carcinoma. PMID: 25226601
    41. The 14-3-3zeta-driven contextual changes of Smad partners from p53 to Gli2 may serve as biomarkers and therapeutic targets of TGF-b-mediated cancer progression. PMID: 25670079
    42. Among the genes found disrupted in this study, there is evidence suggesting that YWHAZ and also the X-linked DRP2 may be considered as novel autism candidate genes. PMID: 23999528
    43. Data found that the interaction between 14-3-3 zeta and Atg9A is mediated by phosphorylation at Ser761. PMID: 25266655
    44. miR-375-mediated regulation of 14-3-3zeta contributes to decrease telomerase activity by altering nuclear translocation of TERT. PMID: 24708873
    45. 14-3-3zeta regulates nuclear trafficking of PP1alpha in mammalian cells PMID: 24956593
    46. By preventing the inactivation of cofilin, metabolic stress-induced degradation of 14-3-3zeta promotes the conversion of blood monocytes into a hypermigratory, proatherogenic phenotype. PMID: 24812321
    47. Compared to HL-60 cells, multidrug-resistant HL-60/VCR cells had increased 14-3-3zeta mRNA and protein expression.Silencing of 14-3-3zeta increased the sensitivity of both sensitive and resistant HL-60 cells to TPT-induced apoptosis. PMID: 24603438
    48. 14-3-3zeta causes synaptic loss by destabilizing microtubules, leading to proteosomal degradation of synaptophysin in the neurons of patients suffering from Alzheimer's disease. PMID: 24367683
    49. Data suggest that the combined expression of 14-3-3zeta and Hsp27 may be a biomarker for predicting survival in patients with NSCLC, and this combination may have potential as a therapeutic target for NSCLC. PMID: 24804299
    50. Somatic copy number alterations by whole-exome sequencing implicates YWHAZ and PTK2 in castration-resistant prostate cancer. PMID: 24114522

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  • 亞細(xì)胞定位：
    Cytoplasm. Melanosome. Note=Located to stage I to stage IV melanosomes.
  • 蛋白家族：
    14-3-3 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12855

    OMIM: 601288

    KEGG: hsa:7534

    STRING: 9606.ENSP00000309503

    UniGene: Hs.492407