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Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

  • 中文名稱:
    人2,3-二磷酸甘油酸(2,3-DPG)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E09861h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人2,3-二磷酸甘油酸(2,3-DPG)酶聯(lián)免疫試劑盒(CSB-E09861h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的2,3DPG含量。2,3-DPG即2,3-二磷酸甘油酸,是紅細(xì)胞糖酵解支路的中間產(chǎn)物。它能調(diào)節(jié)血紅蛋白對(duì)氧氣的親和力,降低其與氧的結(jié)合力,促進(jìn)氧氣釋放。在貧血、高原適應(yīng)等過(guò)程中發(fā)揮重要作用。當(dāng)前對(duì)其在相關(guān)疾病機(jī)制及治療靶點(diǎn)方面有一定研究。試劑盒檢測(cè)范圍為0.312 μmol/mL-20 μmol/mL,適用于基礎(chǔ)研究中紅細(xì)胞代謝功能評(píng)估、缺氧生理機(jī)制探索以及血液系統(tǒng)疾病模型的機(jī)制分析,例如貧血、高原反應(yīng)或心血管相關(guān)疾病的體外實(shí)驗(yàn)研究,滿足細(xì)胞能量代謝、運(yùn)動(dòng)生理學(xué)或病理狀態(tài)下的氧運(yùn)輸調(diào)控等科研需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫:
    2,3DPG
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍:
    0.312 μmol/mL-20 μmol/mL
  • 靈敏度:
    0.078 μmol/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 92-100  
    1:2 Average % 98  
    Range % 92-103  
    1:4 Average % 95  
    Range % 90-99  
    1:8 Average % 90  
    Range % 85-96  
  • 回收率:
    The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 84 80-89  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µmol/ml OD1 OD2 Average Corrected  
    20 2.873 2.712 2.793 2.636  
    10 2.265 2.149 2.207 2.050  
    5 1.372 1.328 1.350 1.193  
    2.5 0.854 0.813 0.834 0.677  
    1.25 0.457 0.481 0.469 0.312  
    0.625 0.337 0.357 0.347 0.190  
    0.312 0.202 0.207 0.205 0.048  
    0 0.161 0.152 0.157    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

我正在研究新的檢測(cè)紅細(xì)胞濃縮物2,3-DPG的方法,我對(duì)你們的ELISA Kit CSB-E09861h很感興趣。如果我想分析一個(gè)普通的EDTA樣本,你是否能夠提供在使用該分析之前使用離心參數(shù)以及Rcf,時(shí)間,溫度等等?還有單次或雙離心周期、周期時(shí)間(s)和離心期間的溫度等?

A:
當(dāng)然可以,您可以直接參考我們的紅細(xì)胞樣品處理方法進(jìn)行檢測(cè)。 抗凝劑的選擇:我們一般使用EDTA,具體方法和要求見(jiàn)樣品制備方法。 我們沒(méi)有檢測(cè)紅細(xì)胞樣品,所以建議您先做預(yù)實(shí)驗(yàn)。