Human 3-nitrotyrosine (3-NT) ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人3-硝基酪氨酸酶聯(lián)免疫試劑盒（CSB-E14324h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的3-NT含量。3-NT（神經營養(yǎng)素-3）是一種由人類NTF3基因編碼的蛋白質，屬于神經生長因子（NGF）家族。它對中樞神經系統(tǒng)和周圍神經系統(tǒng)的特定神經元有調控活性，支持神經細胞的生存和分化，并促進新神經元的生長和分化。3-NT通過與其受體TrkC和TrkB結合，激活細胞信號傳導，影響神經發(fā)生和神經元功能。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外研究領域，如氧化應激相關疾病模型的分子機制探索、抗氧化藥物篩選及藥效評估等實驗場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

3-NT
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 3-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	90
	Range %	82-98
1:4	Average %	104
	Range %	99-111
1:8	Average %	86
	Range %	82-96

回收率：

The recovery of human 3-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	91	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.