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中文名稱:人3-羰基-5-α類固醇4脫氫酶1(SRD5A1)酶聯(lián)免疫試劑盒
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貨號:CSB-EL022653HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人3-羰基-5-α類固醇4脫氫酶1(SRD5A1)酶聯(lián)免疫試劑盒(CSB-EL022653HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的SRD5A1含量。SRD5A1是一種重要靶點。它參與雄激素代謝,可將睪酮轉(zhuǎn)化為活性更強的雙氫睪酮。在脫發(fā)、良性前列腺增生等疾病進程中發(fā)揮作用。對其研究機制主要聚焦于開發(fā)抑制該靶點活性的藥物,以調(diào)節(jié)體內(nèi)雄激素水平,緩解相關(guān)病癥。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于細胞生物學(xué)、分子機制研究及疾病模型構(gòu)建等科研領(lǐng)域,如探究激素代謝通路調(diào)控、藥物靶點篩選或SRD5A1在腫瘤微環(huán)境中的作用分析。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:SRD5A1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
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縮寫:SRD5A1
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Neuroscience
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-102 1:2 Average % 90 Range % 84-95 1:4 Average % 101 Range % 95-106 1:8 Average % 98 Range % 92-104 -
回收率:
The recovery of human SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-93 EDTA plasma (n=4) 92 87-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.017 2.063 2.040 1.795 5 1.499 1.472 1.486 1.241 2.5 1.004 1.064 1.034 0.789 1.25 0.705 0.742 0.724 0.479 0.625 0.501 0.536 0.519 0.274 0.312 0.412 0.438 0.425 0.180 0.156 0.347 0.361 0.354 0.109 0 0.241 0.249 0.245 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Targeting progesterone metabolism in breast cancer with L-proline derived new 14-azasteroids Jyotsana Singh.et al,Bioorganic & Medicinal Chemistry,2017
相關(guān)產(chǎn)品
靶點詳情
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功能:Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
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基因功能參考文獻:
- The study described in this article shows that selected SRD5A1 and SRD5A2 polymorphisms can alter the levels of metabolic and hormonal parameters in patients with BPH. Special attention should be paid to the SDR5A2 rs12470143 polymorphism, which is associated with a change in lipid profile, as well as with the inheritance and incidence rate of MetS among these patients. PMID: 29084161
- Findings suggest that 5alpha-reductases (5-AR) isoenzymes could be explored as a therapeutic target for urothelial bladder cancer (UBC) with 5alpha-reductase inhibitors (5-ARI). PMID: 29187470
- we demonstrated that AR, 5alphaR1 and 5alphaR2 immunoreactivity in bladder urothelial carcinoma cells significantly decreased with advanced tumor grade. PMID: 28947209
- Studies indicate that enhanced 5alpha-reductase activity in subjects with polycystic ovary syndrome (PCOS) was associated with insulin resistance (IR) rather than obesity. PMID: 28347315
- Although 4-dione is the main source of 5alpha-dihydrotestosterone in human preadipocytes, production of this steroid by 5 alpha-reductase isoenzymes (SRD5A1, 2 and 3) mediates the inhibitory effect of both 4-dione and testosterone on preadipocyte differentiation. PMID: 26855069
- SRD5A1 small-interfering RNA knockdown led to significant increase in progesterone receptor-B nuclear import, ectopic, whereas SRD5A1 overexpression resulted in remarkable inhibition of nuclear progesterone receptor-B in P4-treated cells. Repression of SRD5A1 activated progesterone receptor-B responsive gene, whereas overexpression of SRD5A1 possessed an inhibitory effect. PMID: 26243543
- the genotypic distribution of 22 single nucleotide polymorphisms in SRD5A1 and SRD5A2 in a set of Spanish prostate cancer patients, were evaluated. PMID: 26174685
- A total of 2 SNPs in SRD5A1-rs3822430 and rs1691053-were associated with prostate-specific antigen level at diagnosis. PMID: 25960412
- study found SRD5A enzyme is present in spermatozoa and that its cellular content is lowered in sperm samples from varicocele patients compared to healthy subjects PMID: 25850410
- Results show that 5alpha-reductase type 1 was associated with intratumoral dihydrotestosterone concentrations and is an independent prognostic factor in endometrioid endometrial adenocarcinoma. PMID: 25475427
- In model cell lines of endometrial cancer, AKR1C2 and SRD5A1 have crucial roles in progesterone metabolism. PMID: 25463305
- Increased 5alphaR activity and glucocorticoid secretion rate over time are linked with the development of metabolic disease, and may represent targets for therapeutic intervention. PMID: 24986533
- SRD5A1 rs166050C risk variant was associated with greater prostatic exposure to androsterone PMID: 24277450
- Significantly higher levels of SRD5A1, AKR1C2, AKR1C3, and HSD17B10 mRNA were however found in bone metastases than in non-malignant and/or malignant prostate tissue PMID: 24244276
- SRD5A1 and SRD5A2 single nucleotide polymorphisms are significantly associated with the clinical characteristics of benign prostatic hyperplasia and the efficacy of benign prostatic hyperplasia treatment. PMID: 23499746
- Different effects were seen for progesterone, which significantly decreased SRD5A1 protein levels. PMID: 23183084
- Postmenopausal breast cancer risk associated with combined therapy may be modified by genetic variation in SRD5A1 PMID: 21947678
- Based on a limited selection of gene variants, no involvement of SRD5A1, SRD5A2, ESR1, ESR2 or PGR in female pattern hair loss is evident. PMID: 22509838
- The different expression levels of 5alpha-reductase isoenzymes may confer response or resistance to 5alpha-reductase inhibitors. PMID: 22194926
- Ten percent of cases and 44% of controls had the cytosine/cytosine (C/C) genotype for the SRD5A1 SNP, rs501999 indicating that this genotype may protect women against severe premenstrual symptoms. PMID: 22273344
- Evidence of association of two alleles for alcohol dependence (AD) is found in SRD5A1 and AKR1C3, mediating a protective effect of the minor allele at each AD marker based on the genotype of the second marker. PMID: 21323680
- Show positive associations of several SRD5A1 and SRD5A2 variations as independent predictors of biochemical recurrence after radical prostatectomy. PMID: 21715084
- Single nucleotide polymorphisms in the two isoforms of 5alpha-reductase, SRD5A1 and SRD5A2, are associated with lean patients with polycystic ovary syndrome. PMID: 21530059
- the dominant route of Dihydrotestosterone synthesis in castration-resistant prostate cancer bypasses testosterone, and instead requires 5alpha-reduction of androstenedione by SRD5A1 to 5alpha-androstanedione, which is then converted to DHT PMID: 21795608
- A statistically significant reduction of SRD5A1, SRD5A2, and CYP19A gene expression was found in the dermal papillae cells and peripheral blood mononuclear cells in idiopathic hirsutism. PMID: 21676395
- Pitfalls in the diagnosis of 5alpha-reductase type 2 deficiency during early infancy. PMID: 21447938
- High nuclear SRD5A1 expression is associated with higher prostate cancer stage. PMID: 20519274
- We investigated associations between single nucleotide polymorphisms in genes HSD3B1, SRD5A1/2, and AKR1C2 and prostate cancer risk PMID: 20056642
- Data indicate that enzymes CYP17A1 and HSD3B1 showed low expression, while AKR1C3 and SRD5A1 were abundantly expressed. PMID: 20086173
- role in blood pressure PMID: 11903314
- examination of differential methylation of isoenzyme genes in lymphocytes PMID: 11948017
- polymorphisms of SRD5A1 and SRD5A2 genes may not be directly associated with the development of baldness or generation of different clinical phenotypes. PMID: 12670724
- Loss of 5alpha-reductase type I expression is associated with metastatic prostate cancer PMID: 12738739
- Changes in 5alpha-reductase, 11beta-HSD1, and 20alpha/beta-HSD enzyme activities observed in polycystic ovary syndrome may contribute to increased production of cortisol and androgens. PMID: 14671189
- Mutant SRD5A1 isoenzyme does not seem to play a crucial role in the development of hypospadias. PMID: 14739525
- The direct challenge of beta-adrenergic agonists to glioma cells resulted in the rapid and transient elevation of 5alpha-R mRNA levels through the activation of the cyclic AMP (cAMP)/protein kinase A-mediated signaling pathway. PMID: 14997014
- Glucocorticoids and androgens differentially regulate 5alphaR1 mRNA in the rat liver. Regulation of 5alphaR1 gene is primarily at the post-transcriptional level. PMID: 15004407
- Candidate gene for benign prostatic hyperplasia. PMID: 15136785
- Expression of SRD5A1 (5alphaR1) and SRD5A2 (5alphaR2) is elevated, and expression of AKR1C1 (20alpha-HSO), AKR1C2 (3alpha-HSO3) and AKR1C3 (3alpha-HSO2) is reduced in tumorous as compared to normal breast tissue. PMID: 15212687
- 5alpha-reductase type 1 (5alphaR1) immunostaining is increased and 5alpha-reductase type 2(5alphaR2) immunostaining is decreased during development of prostate cancer and there is increased expression of both in recurrent and metastatic cancers PMID: 15538746
- Serenoa repens inhibits this enzyme in the prostate, but does not suppress prostate-specific antigen secretion in prostate cancer. PMID: 15543614
- 5alpha-reductase is expresed in human embryonic kidney cell line HEK293 PMID: 15792368
- The expression of 5alpha-reductase type I mRNA was not regulated by calcitriol in a prostate tumor cell line. PMID: 15862960
- SRD5A1 is abundantly transcribed in normal human genital skin fibroblasts and may play an important role in masculinization of SRD5A2-deficient males. PMID: 15941927
- The SRD5A1 mRNA expression is 2-fold lower than SRD5A2 in normal rat. PMID: 16818707
- These results suggest that intratumoral 5 alpha-dihydrotestosterone concentration is mainly determined by 5alphaRed1 and aromatase in breast carcinoma tissues. PMID: 17066438
- Both 5-alpha-reductase I and II are expressed in normal human prostate stromal and epithelial cells. Only 5-alpha-reductase isoenzymes of prostate epithelium are modulated by oxytocin. PMID: 18008328
- Hepatocyte growth factor stimulates 5alpha-R1 expression through up-regulation of the transcription factor early growth response 1 PMID: 18215136
- Our results demonstrate that the 5alphaR activities of either bDPCs or sDPCs are stronger than that of dermal fibroblasts, despite the fact that the major steroidogenic activity is attributed to 17beta-HSD rather than 5alphaR among the three cell types. PMID: 18258185
- Testosterone 5-alpha-Reductase deficiency is associated with gender identity PMID: 18422030
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亞細胞定位:Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
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蛋白家族:Steroid 5-alpha reductase family
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組織特異性:Liver and prostate (at a low level).
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