Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

產(chǎn)品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人5羥二十碳四烯酸(5-HETE)酶聯(lián)免疫試劑盒（CSB-E17006h）檢測血清、血漿樣本中的5-HETE含量。5-HETE即5-羥二十碳四烯酸，是花生四烯酸經(jīng)5-脂氧合酶代謝的產(chǎn)物。它在炎癥、過敏等病理過程中扮演重要角色。研究機制主要圍繞其在細胞信號傳導中的作用，能調(diào)節(jié)免疫細胞活性、促進血管生成等，是藥物研發(fā)潛在靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本產(chǎn)品適用于體外定量分析生物樣本中5-HETE的表達水平，為研究炎癥相關疾病機制、評估藥物干預效果或探索代謝通路調(diào)控提供可靠的實驗工具，廣泛應用于生命科學基礎研究、藥理學研究及生物標志物開發(fā)等領域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

5-HETE
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HETE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
Range %	84-94
1:200	Average %	92
Range %	87-99
1:400	Average %	93
Range %	85-97
1:800	Average %	97
Range %	90-104

回收率：

The recovery of human 5-HETE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	91	84-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.