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  4. Human 6-hydroxymelatonin sulfate (6HMS) ELISA kit

Human 6-hydroxymelatonin sulfate (6HMS) ELISA kit

  • 中文名稱：
    人6-羥基硫酸褪黑素(6HMS)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EQ027870HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人6-羥基硫酸褪黑素(6HMS)酶聯(lián)免疫試劑盒（CSB-EQ027870HU）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的6HMS含量。人6-羥基硫酸褪黑素（6HMS）是褪黑素的代謝產(chǎn)物，參與調(diào)節(jié)生物節(jié)律和抗氧化作用。研究發(fā)現(xiàn)，6HMS在妊娠期高血壓疾病（HDCP）中發(fā)揮重要作用，通過調(diào)節(jié)胎盤氧化應(yīng)激水平和內(nèi)皮功能，參與HDCP的發(fā)病機制。試劑盒檢測范圍為10 pg/mL- 400 pg/mL，可應(yīng)用于褪黑素代謝通路研究、神經(jīng)內(nèi)分泌系統(tǒng)功能分析，或探索6HMS在睡眠障礙、神經(jīng)退行性疾病及免疫調(diào)節(jié)中的潛在作用機制，為體外實驗提供穩(wěn)定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    6HMS
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    10 pg/mL- 400 pg/mL
  • 靈敏度：
    10 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human 6HMS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 92-98  
    1:2 Average % 95  
    Range % 87-105  
    1:4 Average % 96  
    Range % 89-102  
    1:8 Average % 92  
    Range % 88-96  
  • 回收率：
    The recovery of human 6HMS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-100  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.154 0.156 0.155    
    200 0.360 0.342 0.351    
    92 0.572 0.543 0.558    
    30 0.856 0.860 0.858    
    10 1.475 1.489 1.482    
    0 2.341 2.435 2.388    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

I would like to know if you recommend using lysis buffer in order to remove the blood from the tissue when running the 6-hydroxymelatonin ELISA KIT in tissue. Our specimens are largely supplied by blood what could mask results.

A:
Thanks for your information.
So sorry that we have no experience on using lysis buffer remove the blood from the tissue.
Anyway,if it helps,we are glad to recommend to you our sample method on tissue stated it as in the manual"100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C"
And if you worry the blood effect, pls use PBS to wash the sample longer.