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  4. Human 8-epi-prostaglandin F2alpha,8-epi-PGF2α ELISA Kit

Human 8-epi-prostaglandin F2alpha,8-epi-PGF2α ELISA Kit

  • 中文名稱：
    人8-異構(gòu)前列腺素F2α(8-epi-PGF2α)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09441h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人8-異構(gòu)前列腺素F2α(8-epi-PGF2α)酶聯(lián)免疫試劑盒（CSB-E09441h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、尿液、組織勻漿樣本中的8-epi-PGF2α含量。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，該產(chǎn)品適用于體外研究樣本中氧化應(yīng)激水平的動(dòng)態(tài)監(jiān)測(cè)，可應(yīng)用于動(dòng)物模型或細(xì)胞實(shí)驗(yàn)中評(píng)估抗氧化治療效果、疾病進(jìn)程中氧化損傷程度等科研場(chǎng)景，為探索氧化應(yīng)激相關(guān)分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    8-epi-prostaglandin F2α,8-epi-PGF2α,8-epi-PGF2 alpha
  • 縮寫：
    8-epi-PGF2α
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, tissue homogenates
  • 檢測(cè)范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human 8-epi-PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %87-103
    1:2Average %86
    Range %82-93
    1:4Average %100
    Range %95-103
    1:8Average %93
    Range %84-100
  • 回收率：
    The recovery of human 8-epi-PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.965 2.897 2.931 2.820
    25002.211 2.323 2.267 2.156
    12501.599 1.566 1.583 1.472
    6250.811 0.802 0.807 0.696
    3120.505 0.474 0.490 0.379
    1560.311 0.301 0.306 0.195
    780.251 0.234 0.243 0.132
    00.113 0.109 0.111 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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