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  4. Human A disintegrin and metalloproteinase with thrombospondin motifs 2(ADAMTS2) ELISA kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 2(ADAMTS2) ELISA kit

  • 中文名稱：
    人金屬肽酶含血小板反應(yīng)蛋白2(ADAMTS2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL001308HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人金屬肽酶含血小板反應(yīng)蛋白2(ADAMTS2)酶聯(lián)免疫試劑盒（CSB-EL001308HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADAMTS2含量。ADAMTS2是一種細胞外金屬蛋白酶，參與細胞外基質(zhì)破壞、血管平滑肌細胞分型與凋亡、炎癥細胞浸潤、氧化應(yīng)激等復(fù)雜機制。其在心臟肥厚、肝纖維化等疾病中發(fā)揮重要作用，通過調(diào)節(jié)PI3K/Akt信號通路影響心肌細胞肥大反應(yīng)。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，支持科研人員探究 ADAMTS2 在皮膚彈性維持、器官纖維化病理機制或腫瘤微環(huán)境調(diào)控中的作用，為疾病模型構(gòu)建、藥物靶點篩選及生物標志物研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    A disintegrin and metalloproteinase with thrombospondin motifs 2 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 2 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 2 ELISA Kit; ADAM TS 2 ELISA Kit; ADAM TS2 ELISA Kit; ADAM-TS 2 ELISA Kit; ADAM-TS2 ELISA Kit; ADAMTS 3 ELISA Kit; ADAMTS-2 ELISA Kit; ADAMTS2 ELISA Kit; ATS2_HUMAN ELISA Kit; EC 3.4.24.14 ELISA Kit; EDS VIIB ELISA Kit; EDS VIIC ELISA Kit; hPCPNI ELISA Kit; NPI ELISA Kit; PC I NP ELISA Kit; PC I-NP ELISA Kit; PCINP ELISA Kit; PCPNI ELISA Kit; pNPI ELISA Kit; Procollagen I N proteinase ELISA Kit; Procollagen I N-proteinase ELISA Kit; Procollagen I/II amino propeptide processing enzyme ELISA Kit; Procollagen I/II amino propeptide-processing enzyme ELISA Kit; Procollagen N endopeptidase ELISA Kit; Procollagen N-endopeptidase ELISA Kit
  • 縮寫：
    ADAMTS2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-95
    1:2 Average % 92
    Range % 88-96
    1:4 Average % 87
    Range % 82-91
    1:8 Average % 95
    Range % 90-100
  • 回收率：
    The recovery of human ADAMTS2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 98-109
    EDTA plasma (n=4) 97 92-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.129 2.238 2.184 2.023
    100 1.737 1.682 1.710 1.549
    50 1.112 1.174 1.143 0.982
    25 0.719 0.741 0.730 0.569
    12.5 0.480 0.459 0.470 0.309
    6.25 0.360 0.350 0.355 0.194
    3.12 0.239 0.247 0.243 0.082
    0 0.159 0.163 0.161 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form with reduced collagen-binding activity.
  • 基因功能參考文獻：
    1. We identified three novel homozygous loss-of-function mutations (c.2927_2928delCT, p.(Pro976Argfs*42); c.669_670dupG, p.(Pro224Argfs*24); and c.2751-2A>T) and one compound heterozygous mutation (c.2T>C, p.? and c.884_887delTGAA, p.(Met295Thrfs26*)) in ADAMTS2 in five patients from four unrelated families PMID: 26765342
    2. Data indicate that ADAMTS2 and 3 cleave the amino-propeptide of fibrillar collagens and regulate blood vessels homeostasis and lymphangiogenesis. Also, ADAMTS2 deficiency leads to the dermatosparactic type of Ehlers-Danlos syndrome. [review] PMID: 25863161
    3. IL-1a is a strong positive regulator of ADAMTS-2 and ADAMTS-3 expression. PMID: 26232334
    4. ADAMTS2 was significantly overexpressed in Fibrous dysplasia (FD) tissues, but rarely expressed in normal bone tissues, suggesting that ADAMTS2 could be a potential biomarker for FD PMID: 25674217
    5. Pathway inhibition studies revealed that ADAMTS-2 upregulation by IL-6 was mediated by JNK pathway. PMID: 24752352
    6. Data show that ADAMTS-2 is able to reduce proliferation of endothelial cells, and to induce their retraction and detachment from the substrate resulting in apoptosis. PMID: 20574651
    7. Determination of the processing, activity and cleavage specificity of the bovine ADAMTS2 protein. PMID: 16046392
    8. when conditioned media of TSP2-transfectants were added to cultures of bovine pulmonary microvascular endothelial cells (BPMEC), the BPMEC proliferation was significantly inhibited; suggesting that human TSP2 is a potential inhibitor of angiogenesis PMID: 11788898
    9. Although exons 3-5 or 14-16 encode protein domains that have not been recognized as crucial for ADAMTS-2 activity, the aminoprocollagen processing was strongly impaired, providing evidence for the requirement of these domains for proper enzyme function PMID: 15373769

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  • 相關(guān)疾?。?/div>
    Ehlers-Danlos syndrome 7C (EDS7C)
  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 218

    OMIM: 225410

    KEGG: hsa:9509

    STRING: 9606.ENSP00000251582

    UniGene: Hs.23871