Human Angiopoietin 2,ANG-2 ELISA Kit
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中文名稱:人血管生成素2(ANG-2)酶聯(lián)免疫試劑盒
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貨號:CSB-E04500h
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人血管生成素2(ANG-2)酶聯(lián)免疫試劑盒(CSB-E04500h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ANGPT2含量。ANGPT2是一種重要靶點(diǎn)。它在血管生成、血管穩(wěn)定等生理過程中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)其可調(diào)節(jié)血管內(nèi)皮細(xì)胞功能,與腫瘤生長、轉(zhuǎn)移及眼部新生血管疾病等病理過程密切相關(guān),對其作用機(jī)制的深入探索有望為相關(guān)疾病治療提供新思路。試劑盒檢測范圍為39 pg/mL-2500 pg/mL,靈敏度為9.75 pg/mL。適用于心血管疾病機(jī)制研究、腫瘤血管生成分析、炎癥相關(guān)動物模型評估等科研領(lǐng)域,尤其適用于需要多維度樣本對比的轉(zhuǎn)化醫(yī)學(xué)研究本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:AGPT 2 ELISA Kit; Agpt2 ELISA Kit; ANG 2 ELISA Kit; ANG-2 ELISA Kit; ANG2 ELISA Kit; Angiopoietin 2a ELISA Kit; Angiopoietin 2B ELISA Kit; Angiopoietin-2 ELISA Kit; Angiopoietin2 ELISA Kit; ANGP2_HUMAN ELISA Kit; ANGPT 2 ELISA Kit; Angpt2 ELISA Kit; Tie2 ligand ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:39 pg/mL-2500 pg/mL
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靈敏度:9.75 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cardiovascular
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
312.245
312.541
312.837
312.659
312.866
312.837
SD
0.033
0.032
0.028
0.042
0.046
0.043
CV(%)
3.905
3.783
3.306
4.962
5.430
5.077
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
89
Range %
83-96
1:2
Average %
95
Range %
91-98
1:4
Average %
95
Range %
88-101
1:8
Average %
98
Range %
91-103
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回收率:
The recovery of human ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
85
80-92
EDTA plasma (n=4)
95
92-101
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2500
2.925
2.960
2.943
2.784
1250
2.547
2.505
2.526
2.367
625
1.656
1.799
1.728
1.569
312.5
0.829
0.888
0.859
0.700
156.25
0.401
0.411
0.406
0.247
78
0.253
0.252
0.253
0.094
39
0.211
0.235
0.223
0.064
0
0.165
0.152
0.159
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Platelet-derived extracellular vesicles promote endothelial dysfunction in sepsis by enhancing neutrophil extracellular traps M Jiang,BMC immunology,2023
- ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway F Chen,Journal of translational medicine,2023
- Serum levels and gene polymorphisms of angiopoietin 2 in systemic lupus erythematosus patients JM Wang,Scientific Reports,2021
- The contribution of dietary and plasma folate and cobalamin to levels of angiopoietin-1, angiopoietin-2 and Tie-2 receptors depend on vascular endothelial growth factor status of primary breast cancer patients Pirouzpanah S, et al,Scientific Reports,2019
- Wound Healing Potential of Low Temperature Plasma in Human Primary Epidermal Keratinocytes Hui Song Cui, et al,Tissue Engineering and Regenerative Medicine,2019
- N-Acetyl-Seryl-Asparyl-Lysyl-Proline interaction with ACE2-Ang (1-7)-Mas axis attenuates pulmonary fibrosis in silicotic rats Xuemin G, et al,Experimental Physiology,2019
- Angiopoietin-1, Angiopoietin-2, and Periostin Levels in Children with Recurrent Wheeze Burcu Tahire Koksal, et al,Pediatric allergy immunology and pulmonology,2018
- Predictive Value of Combined LIPS and ANG-2 Level in Critically Ill Patients with ARDS Risk Factors Zhi Xu.et al,Mediators of Inflammation,2018
- Predictive value of combined LIPS and ANG-2 in critically ill patients with ARDS risk factors Zhi Xu.et al,/,/
- The diagnostic and prognostic value of angiopoietins compared with C-reactive protein and procalcitonin in children with febrile neutropenia Esra Mimaroglu.et al,Turk J Pediatr.,2017
- Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia?inducible factor 1α in human dermal fibroblasts Hui SongCui.et al,Archives of Biochemistry and Biophysics,2017
- Angiogenic factors are associated with development of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation Nie DM. et al,J Huazhong Univ Sci Technolog Med Sci,2015
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:ANGPT2也是一個重要的血管生成調(diào)節(jié)因子,與ANGPT1形成對比。它可以增強(qiáng)血管通透性和炎癥反應(yīng),并在多種疾病中發(fā)揮重要作用。最近的研究表明,抑制ANGPT2可能有助于治療腫瘤、心血管疾病和炎癥性疾病等疾病。
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功能:Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
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基因功能參考文獻(xiàn):
- plasma ANG-2 level, the acute physiology and chronic health evaluation 2 (APACHE2) score and lung injury prediction score are correlated with acute respiratory distress syndrome PMID: 30008611
- Study is the first to demonstrate that Ang2 is upregulated not only in the local skin lesions but also in the systemic circulation, and that its serum levels correlate well with disease severity in psoriasis vulgaris patients. PMID: 28497874
- regulatory mechanisms of Ang-2 in NSCLC PMID: 30454550
- This study demonstrates that Ang-2 levels are significantly upregulated in sepsis-associated coagulopathy (SAC), and this biomarker can be used to risk stratify patients with sepsis into non-overt disseminated intravascular coagulation (DIC) and overt DIC. PMID: 29996658
- Flunarizine protected ECs from TNFalpha-induced increase in Angpt-2 transcription and vascular barrier breakdown. PMID: 28276491
- Study findings delineate the existence of the beta-estradiol (E2)-ANGPT2 axis in hair follicles and suggest that, by substituting E2, ANGPT2 may provide a novel strategy for the treatment of postmenopausal female pattern hair loss. PMID: 29724581
- Results suggest that hCPFs-Exo transports low expressed exosomal miR-106b-5p to endothelial cells and promotes angiogenesis by overexpression of Angpt2. PMID: 29905392
- Angpt2 is an independent predictor of adverse clinical outcomes in diabetic patients. Further studies are needed to identify the pathogenic role of Angpt2 in renal deterioration and cardiovascular complications of diabetes mellitus. PMID: 29642068
- High ANG2 expression is associated with Acute respiratory distress syndrome. PMID: 30171880
- PVT1 was able to bind and degrade miR26b to promote connective tissue growth factor (CTGF) and angiopoietin 2 (ANGPT2) expression. PMID: 29620147
- Serum Ang-2 may be a useful tumor marker in predicting liver cancer prognosis. PMID: 29253494
- High ANG2 expression is associated with angiogenesis in breast cancer. PMID: 28534941
- Angiopoietin-2 acts as a survival factor for chronic lymphocytic leukemia B cells throughout Tie-2 receptor engagement. PMID: 28580615
- serum level elevated in pre-eclampsia, not significantly affected by HIV status PMID: 28627965
- Plasma levels of Ang2 were associated with markers of malaria severity and levels of var transcripts encoding P. falciparum Erythrocyte Membrane Protein 1 (PfEMP1) containing Cysteine Rich Inter Domain Region alpha1 (CIDRalpha1) domains predicted to bind Endothelial Protein C receptor (EPCR). PMID: 27784899
- Hepatitis C virus induces Ang2 expression in hepatocytes. PMID: 28027429
- the relationship between lung cancer stage and Ang 2 was documented with this study and the expression rate was found to be lower in adenocarcinomas. By this analysis, we can suggest that angiopoietins may be used as an option for targeted treatment in lung cancer. PMID: 27811442
- Endothelial glycocalyx breakdown is mediated by Angpt-2 in a non-redundant manner. PMID: 28453727
- The relation between angiopoietin-2 and assessed parameters of vascular structure in type 1 diabetes reflects a state of endothelial injury and highlights the role of disturbed angiogenesis and vascular inflammation in the occurrence of diabetic complications. PMID: 27236773
- Results show that MiR-93 targets Ang2 3' UTR and regulates its expression in lung adenocarcinoma. PMID: 28401709
- We demonstrate that ANGPT2 signaling activated after estrogen depletion paradoxically triggers ER+ tumor cell awakening from dormancy in their BM niche, partly indirectly via endothelial Tie2 receptor and partly directly via tumor cell surface integrin &1. PMID: 27353038
- ANG2 did not affect apoptosis or the cell cycle. In contrast, in the in vivo system, overexpression of ANG2 increased tumor growth. PMID: 27492854
- the optimal discrimination cut-off for each cytokine: sVEGFR-1 (2124.5pg/mL), IL-6 (40.2pg/mL), VEGF-A (1060.1pg/mL), Angiopoeintin-2 (913.7pg/mL), uPA (248.1pg/mL), sHER-2/neu (5010pg/mL) and PLGF (93.4pg/mL). For the very first time, a novel cytokine profile associated with cancer metastasis to the paratracheal lymph nodes were reported. PMID: 27599390
- Gab1/SHP2/p38MAPK signaling pathway and Ang-2 have an essential role in regulating thrombin-induced monocyte adhesion and vascular leakage PMID: 27241812
- This study demonstrated that ANGPT2 higher expression in glioblastoma. PMID: 27633774
- These observations provide the first evidence for positive regulation of osteogenesis by ANGPT2 in vitro. PMID: 28214341
- in vitro is has also been shown that Angpt-2 can act as a dose-dependent Tie2 agonist/antagonist. PMID: 27038015
- Ang-2 and sTie-2 plasma levels are increased in pediatric OSA and obesity, particularly when endothelial dysfunction or insulin resistance is detectable. PMID: 28474375
- pathways for two distinctive secretory mechanisms, constitutive and stimulated, of Ang-2 in pulmonary microvascular endothelial cells. PMID: 27585839
- High ANG2 expression is associated with angiogenesis and metastasis via IGF1-IGF1R signaling in epithelial ovarian cancer. PMID: 28898232
- Our novel noninvasive liver fibrosis model, based on serum angiopoietin-2 levels, outperforms other indices and should help substantially in managing CHC and monitoring long-term follow-up prognosis PMID: 23823085
- Tie1 directly interacts with Tie2 to promote ANG-induced vascular responses under noninflammatory conditions, whereas in inflammation, Tie1 cleavage contributes to loss of ANG2 agonist activity and vascular stability PMID: 27548530
- This systematic review and meta-analysis suggested that serum Ang-2 levels might be a potential predictor for staging, and were associated with prognosis of lung cancer. PMID: 28906403
- Studied angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) within the intervertebral disc (IVD) and elucidated their functions in the regulation of nucleus pulposus (NP) cells. Found the ratio of Ang-2/Ang-1 in tissues from patients increased markedly with increasing age and level of degeneration of the IVD. Results indicate Ang-2 plays a role in suppressing cell adhesion&viability, and promotes the apoptosis of NP cells. PMID: 28394321
- Analyses of human gastric cancer tissues showed a strong correlation between DARPP-32 and ANGPT2. The role of DARPP-32-STAT3 axis in regulating ANGPT2 in cancer cells to promote angiogenesis and tumorigenesis. PMID: 25779598
- ANGPT2 expression was upregulated in cerebral cavernous malformation lesions. PMID: 27548575
- Linkage analysis identified a peak (LOD = 4.29) on chromosome 8p23. Follow-up association analysis identified two haplotypes in angiopoietin-2 (ANGPT2) that significantly contributed to the variation of SaO2 (P = 8 x 10-5) and accounted for a portion of the linkage evidence. PMID: 27798093
- Blood ANGPT2 was significantly higher in chronic hepatitis C patients with liver fibrosis compared to those without fibrosis. PMID: 27930387
- This study reveals ANGPT2 as a new susceptibility gene for nAMD and PCV, and it may affect disease susceptibility in association with CFH. PMID: 28192798
- These results underscore a pivotal role of Kaposi's sarcoma-associated herpesvirus -induced Ang-2 in KS tumor development by promoting both angiogenesis and inflammation. PMID: 27294705
- Data suggest that the associations among angiopoietin-2, sFlt-1, coagulation abnormalities and severe course of acute pancreatitis (AP) might be mediated by other bioactive compounds. PMID: 28368336
- An imbalance in ANGPT-2, combined with related factors such as VEGF, beta-catenin, and MMP-2, may partially explain the structural derangements of the arterial wall in patients with chronic obstructive pulmonary disease. PMID: 26801565
- Results demonstrate that Ang-2 expression significantly correlated with poor prognosis for patients with non-small cell lung cancer. [meta-analysis] PMID: 27589869
- Our results show that miR-181a is down-regulated in glioblastoma multiforme (GBM) patients. The three target genes, ANGPT2, ARHGAP18 and LAMC1, are negatively correlated with the expression of miR-181a. Moreover, high expression of ANGPT2 or LAMC1 together with large size of GBM is correlated with a shorter median overall survival PMID: 27176932
- These results suggested that IL-35 restrains rheumatoid arthritis angiogenesis and inflammation by downregulating basal and VEGF-induced Ang2 secretion as well as disrupting Ang2/Tie2 signal transduction. PMID: 27960151
- This study identified Ang-2 as an endothelial cell-derived regulator of BBB permeability. PMID: 26932603
- Serum Ang-2 may be a relevant predictor of Acute Pancreatitis severity, in particular of the development of AP-renal syndrome. PMID: 27022209
- Data show that angiopoietin-2 (Ang-2) and bacterial endotoxins (LPS) follow opposite kinetics in serum in acute pyelonephritis. PMID: 26844659
- IL-19 and Ang-2 might be involved in angiogenesis of T2 Diabetes mellitus complications. PMID: 26657726
- Compared with patients with heart failure or those with orthotopic heart transplantation, serum levels and endothelial expression of Ang-2 were higher in LVAD patients. Ang-2 may contribute to arteriovenous malformation formation and subsequent bleeding in LVAD patients. PMID: 27354285
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