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  4. Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

  • 中文名稱：
    人血管緊張素轉(zhuǎn)化酶2(ACE2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04489h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人血管緊張素轉(zhuǎn)化酶2(ACE2)酶聯(lián)免疫試劑盒（CSB-E04489h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的ACE2含量。ACE2（血管緊張素轉(zhuǎn)換酶2）是一種位于人體細(xì)胞表面的膜蛋白，作為SARS-CoV-2病毒的主要受體，其與病毒表面的S蛋白結(jié)合是病毒進(jìn)入細(xì)胞的關(guān)鍵。研究顯示，ACE2在SARS-CoV-2感染中起重要作用，其動態(tài)表達(dá)變化可能與COVID-19的發(fā)病機(jī)制相關(guān)，且ACE2及其信號通路上的關(guān)鍵分子在肺部炎癥發(fā)生及修復(fù)中發(fā)揮重要作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于體外實(shí)驗(yàn)研究，可應(yīng)用于心血管疾病模型、病毒感染機(jī)制探索、藥物靶點(diǎn)驗(yàn)證等科研場景，例如通過檢測細(xì)胞培養(yǎng)上清中ACE2的動態(tài)變化研究病毒入侵機(jī)制，或分析不同病理?xiàng)l件下生物液體樣本中ACE2的表達(dá)水平差異。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ACE 2 ELISA Kit; ACE related carboxypeptidase ELISA Kit; ACE-related carboxypeptidase ELISA Kit; ACE2 ELISA Kit; ACE2_HUMAN ELISA Kit; ACEH ELISA Kit; Angiotensin converting enzyme 2 ELISA Kit; Angiotensin converting enzyme homolog ELISA Kit; Angiotensin converting enzyme like protein ELISA Kit; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 ELISA Kit; Angiotensin I converting enzyme 2 ELISA Kit; Angiotensin-converting enzyme homolog ELISA Kit; DKFZP434A014 ELISA Kit; EC 3.4.17 ELISA Kit; metalloprotease MPROT 15 ELISA Kit; Metalloprotease MPROT15 ELISA Kit; OTTHUMP00000022963 ELISA Kit; Processed angiotensin-converting enzyme 2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %80-99
    1:2Average %97
    Range %90-105
    1:4Average %95
    Range %91-107
    1:8Average %93
    Range %80-98
  • 回收率：
    The recovery of human ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-98
    EDTA plasma (n=4)9790-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.216 2.182 2.199 1.955
    52.012 1.916 1.964 1.720
    2.51.754 1.643 1.699 1.455
    1.251.326 1.290 1.308 1.064
    0.6251.008 0.944 0.976 0.732
    0.3120.714 0.676 0.695 0.451
    0.1560.416 0.392 0.404 0.160
    00.249 0.239 0.244 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human AE2 antibody.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled ACE2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

該試劑盒（CSB-E04489h）可檢測多少個樣本？

A:
以96T為例，該試劑盒包含8個標(biāo)準(zhǔn)品。若標(biāo)準(zhǔn)品和待測樣品設(shè)為單孔，則該試劑盒可以檢測88個樣品。若標(biāo)準(zhǔn)品設(shè)為復(fù)孔，待測樣品設(shè)為單孔，則該試劑盒可以檢測80個樣品。若待測樣品和標(biāo)準(zhǔn)品均設(shè)為復(fù)孔，則該試劑盒可以檢測40個樣品。

靶點(diǎn)詳情

  • 功能：
    Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, Non-functional as a carboxypeptidase.; (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.; (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
  • 基因功能參考文獻(xiàn)：
    1. Report no influence of ACE2 gene polymorphism on stroke recurrence and only find possible interaction between hypertension history and the ACE2 gene in male stroke patients. PMID: 30056001
    2. In a Chinese Han population, five single-nucleotide polymorphisms (SNP) (rs1514283, rs4646155, rs4646176, rs2285666, and rs879922) in ACE2 gene were determined to significantly associate with essential hypertension in female participants, while no SNP locus was linked to male group. PMID: 30335025
    3. ACE2 and other enzymes can form ANG-(1-7) directly or indirectly from either the decapeptide ANG I or from ANG II. [review] PMID: 29351514
    4. Suggest a potential role for ACE2 polymorphism in postexercise hypotension in hypertensive medicated individuals. PMID: 27925380
    5. ACE2 SNPs and haplotypes are associated with circulating angiotensin-(1-7) levels. PMID: 28895159
    6. we found an interaction between ACE2 and AGTR1 in structuralatrial fibrillation patients in a Chinese Han population PMID: 29441892
    7. results suggest that ACE2, TNNI3K and CALM3 polymorphisms are associated with increased risk of hypertrophic cardiomyopathies and dilated cardiomyopathies and may act as disease modifiers of these diseases. PMID: 28744816
    8. Elevated plasma ACE2 is significantly associated with more advanced LA structural remodeling in atrial fibrillation. PMID: 27738071
    9. Overexpression of ACE2 in monocytes led to reduced endothelial adhesion, transmigration and downregulation of adhesion-related molecules and results in atherosclerosis. PMID: 28186543
    10. These results indicated that aberrant methylation of the ACE2 promoter may be associated with EH risk. In addition, sex may significantly influence ACE2 methylation. PMID: 28440441
    11. ACE2 rs2106809 is an important predictive factor of the response to antihypertensive treatment with ACE inhibitors in Chinese female hypertensive patients. PMID: 27121444
    12. In islets from db/db mice, ACE2 over-expression increased intracellular calcium influx and restored impaired mitochondrial oxidation, potentially causing an increase in GSIS. These results shed light on the potential roles of ACE2 in mitochondrial metabolism, moreover, may improve our understanding of diabetes. PMID: 29128354
    13. ACE2 may have a role in silent atherosclerosis in patients with chronic kidney disease; it counterbalances the vasoconstrictor adverse effects of angiotensin II by its conversion PMID: 27615597
    14. This study reveals an elevated serum concentration of ACE2 and independent associations between serum ACE2 and echocardiographic parameters in hypertensive patients. PMID: 28223093
    15. The findings of this study indicate that ACE-2 activity is reduced in AD and is an important regulator of the central classical ACE-1/Ang II/AT1R axis of renin-angiotensin system, and also that dysregulation of this pathway likely plays a significant role in the pathogenesis of Alzheimer's disease. PMID: 27884212
    16. Overexpression of ACE2 ameliorates Abeta-induced inflammatory response by activating the ACE2/Ang-(1-7)/Mas axis in human RPE cells. PMID: 28605813
    17. Although further studies are required to determine how clusterin suppresses non-specific cellular uptake in phagocytes, our data suggest that clusterin plays a key role in the stealth effect of not only pegylated nanoparticles but also non-pegylated nanoparticles. PMID: 27983983
    18. The ACE2 G8790A polymorphism in type 2 diabetes mellitus patients was correlated with cerebral stroke, and the A allele might be a risk factor of type 2 diabetes mellitus combined with cerebral stroke. PMID: 27500554
    19. ollectrin, an ACE2 homolog with no catalytic activity, regulates blood pressure through an NO-dependent mechanism. Large body of experimental data confirmed sustained beneficial effects of ACE2/Ang-(1-7)/Mas receptor axis activation on hypertension and vascular injury. PMID: 27889958
    20. The potential contribution of the ACE2 to cardiovascular disease progression was addressed. PMID: 27965422
    21. Serum ACE2 activity was significantly lower in acute ischemic stroke as compared to both control and stroke-alert patients, followed by an increase to control levels at three days. PMID: 27488276
    22. Here, we review the role and effects of ACE2, ACE2 activators, Ang-(1-7) and synthetic Mas receptor agonists in the control of inflammation and fibrosis in cardiovascular and renal diseases and as counter-regulators of the ACE-Ang II-AT1 axis. PMID: 26995300
    23. ACE2 overexpression inhibited cell growth. PMID: 27460845
    24. Downregulation of ACE2/Ang-(1-7)/Mas axis stimulates breast cancer metastasis through the activation of store-operated calcium entry and PAK1/NF-kappaB/Snail1 pathways. PMID: 27063099
    25. The circulating ACE2 and Ang-(1-7) levels were related to neither rs4646155 nor rs879922 in female or male patients.In conclusion, the rs2106809 polymorphism of the ACE2 gene may be a determinant of the circulating Ang-(1-7) level in female patients with hypertension, suggesting a genetic association between circulating Ang-(1-7) levels and ACE2 gene polymorphisms in patients with hypertension. PMID: 27310975
    26. These results indicated that angiotensin-(1-7)/ACE2/Mas axis may reduce liver lipid accumulation partly by regulating lipid-metabolizing genes through ATP/P2 receptor/CaM signaling pathway. PMID: 26883384
    27. ACE-2 significantly increased when IMR-90 were hypoxic prior to hyperoxic exposure with no recovery. PMID: 27093376
    28. Imbalanced down-regulation of ACE and ACE2 mRNA expression levels may play an important role in the development and progression of thoracic aortic aneurysmal dilatation and subsequently dissection. PMID: 25237166
    29. These results suggest that the ACE2 G8790A and rs2106809 polymorphisms may be associated with essential hypertension risk. PMID: 25237167
    30. ACE and ACE2 expression at the mRNA and protein levels are significantly increased in the myocardium of patients with heart failure. PMID: 25869724
    31. Multivariable regression analysis revealed that urinary L-FABP and urinary albumin/ creatinine ratio were significantly associated with urinary ACE2 levels. PMID: 26067610
    32. ACE2 and Ang-(1-7) significantly inhibit early atherosclerotic lesion formation via protection of endothelial function and inhibition of inflammatory response. PMID: 25721616
    33. ACE-2 is expressed in fetal human lung fibroblasts but is significantly decreased by hyperoxic gas PMID: 25665060
    34. urinary ACE2 increased in type 2 diabetic patients with various degrees of albuminuria PMID: 25791940
    35. soluble ACE2 is involved in the pathomechanism of hypertension and heart failure. PMID: 24691269
    36. Decrease in circulating ACE2 activity was associated with cardiovascular disease in patients with chronic kidney disease. PMID: 25813276
    37. By genetic replenishment of ACE2 and pharmaceutical use of ARB, restored ACE2 level mitigated GBC growth. Our results supported the rationale for the use of ARB in GBC patients for potential therapeutic benefit PMID: 25663464
    38. There is no clear association between ACE2 gene A9570G polymorphisms and childhood primary nephrotic syndrome. PMID: 25815490
    39. Our results revealed that SNPs rs2074192 and rs714205 in ACE2 gene were associated with the susceptibility of DR and PDR. PMID: 25359286
    40. Olmesartan may uniquely increase urinary ACE2 level in hypertensive patients, which could potentially offer additional renoprotective effects. PMID: 24842388
    41. Hepatocellular carcinoma patients with higher level of ACE2 expression had longer survival time than those with lower level of ACE2 expression. PMID: 25701390
    42. Urinary ACE2 activity and protein expression are increased in type 1 diabetes patients prior to the onset of clinical complications. PMID: 24920267
    43. Brain endoplasmic reticulum stress does not contribute to DOCA-salt hypertension and ACE2 blunts neurogenic hypertension independently of ER stress. PMID: 25519733
    44. Partial loss of ACE2 is sufficient to enhance the susceptibility to heart disease. PMID: 24728465
    45. These data demonstrate that MSCs modified to overexpress the ACE2 gene can produce biologically active ACE2 protein over a sustained period of time and have an enhanced ability to promote endothelial repair after LPS challenge PMID: 25200929
    46. ACE2 cleavage is regulated by influenza A H1N1 neuraminidase. PMID: 24662240
    47. among females ACE2 rs2106809 polymorphisms,and among males ACE2 rs2106809 polymorphism and alcohol consumption are associated with essential hypertension PMID: 24112034
    48. Data suggest angiotensin (ANG) converting enzyme 2/ANG-II-(1-7)/proto-oncogene protein Mas axis regulates inflammation/fibrosis, cell proliferation, and leukocyte recruitment/activation; main topic here is kidney/inflammatory renal disease. [REVIEW] PMID: 23488800
    49. ACE2 and FZD1 are prognosis markers in squamous cell/adenosquamous carcinoma and adenocarcinoma of gallbladder. PMID: 23921915
    50. ACE2 overexpression in the rostral ventrolateral medulla attenuates the enhanced tonically active glutamatergic input in SHRs, which may be an important mechanism underlying the beneficial effect of central ACE2 to hypertension. PMID: 24838502

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  • 亞細(xì)胞定位：
    [Processed angiotensin-converting enzyme 2]: Secreted.; Cell membrane; Single-pass type I membrane protein. Cytoplasm. Cell projection, cilium. Apical cell membrane.; [Isoform 2]: Apical cell membrane.
  • 蛋白家族：
    Peptidase M2 family
  • 組織特異性：
    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in the renal proximal tubul
  • 數(shù)據(jù)庫鏈接：

    HGNC: 13557

    OMIM: 300335

    KEGG: hsa:59272

    STRING: 9606.ENSP00000252519

    UniGene: Hs.178098