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  4. Human Apolipoprotein C-IV(APOC4) ELISA kit

Human Apolipoprotein C-IV(APOC4) ELISA kit

  • 中文名稱：
    人載脂蛋白C-IV(APOC4)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL001934HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人載脂蛋白C-IV(APOC4)酶聯(lián)免疫試劑盒（CSB-EL001934HU）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOC4含量。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，科研人員可將其應(yīng)用于脂代謝異常疾病模型構(gòu)建、基因調(diào)控機(jī)制探索或生物樣本中APOC4表達(dá)水平評(píng)估等實(shí)驗(yàn)場景，為分子機(jī)制研究提供定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    APO C4 ELISA Kit; Apo CIV ELISA Kit; Apo-CIV ELISA Kit; APOC 4 ELISA Kit; ApoC IV ELISA Kit; ApoC-IV ELISA Kit; APOC4 ELISA Kit; APOC4_HUMAN ELISA Kit; Apolipoprotein C IV ELISA Kit; Apolipoprotein C-IV ELISA Kit; Apolipoprotein C4 ELISA Kit
  • 縮寫：
    APOC4
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:200Average %92
    Range %88-96
    1:400Average %87
    Range %84-90
    1:800Average %95
    Range %91-99
    1:1600Average %97
    Range %92-102
  • 回收率：
    The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9894-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    4000.080 0.083 0.082
    2000.117 0.119 0.118
    1000.177 0.172 0.175
    500.276 0.296 0.286
    250.504 0.490 0.497
    12.50.923 0.931 0.927
    6.251.461 1.496 1.479
    02.539 2.587 2.563
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May participate in lipoprotein metabolism.
  • 基因功能參考文獻(xiàn)：
    1. APOC4 rs1132899 polymorphism was associated with an increased risk of premature coronary artery disease in Chinese subjects. PMID: 26129832
    2. ApoC-IV overexpression may perturb lipid metabolism leading to lipid accumulation. HCV core protein may modulate ApoC-IV expression through Ku antigen and PPARgamma/RXRalpha complex. PMID: 18809223
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein C4 family
  • 組織特異性：
    Expressed by the liver and secreted in plasma.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 611

    OMIM: 600745

    KEGG: hsa:346

    STRING: 9606.ENSP00000468236

    UniGene: Hs.731599