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  4. Human Apolipoprotein C2,apo-C2 ELISA Kit

Human Apolipoprotein C2,apo-C2 ELISA Kit

  • 中文名稱：
    人載脂蛋白C2(apo-C2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14174h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人載脂蛋白C2(apo-C2)酶聯(lián)免疫試劑盒（CSB-E14174h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOC2含量。APOC2是重要靶點。它是載脂蛋白C-Ⅱ，在脂質(zhì)代謝中起關(guān)鍵作用。研究機制表明，APOC2能激活脂蛋白脂肪酶，促進(jìn)乳糜微粒和極低密度脂蛋白分解代謝。對其深入研究，或為高脂血癥等脂質(zhì)相關(guān)疾病治療提供新方向。試劑盒檢測范圍為31.2 ng/mL-2000 ng/mL，該產(chǎn)品可廣泛應(yīng)用于基礎(chǔ)醫(yī)學(xué)研究中apo-C2表達(dá)水平的動態(tài)監(jiān)測，例如探究脂代謝調(diào)控機制、評估心血管疾病動物模型干預(yù)效果，或結(jié)合組織樣本分析apo-C2在特定器官中的分布特征。其寬泛的樣本兼容性為體外實驗與離體研究提供靈活選擇，適用于細(xì)胞實驗、病理模型構(gòu)建及生物標(biāo)志物篩查等科研場景，為脂蛋白相關(guān)領(lǐng)域研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    APC 2 ELISA Kit; APC2 ELISA Kit; Apo CII ELISA Kit; Apo-CII ELISA Kit; APOC 2 ELISA Kit; ApoC II ELISA Kit; ApoC-II ELISA Kit; APOC2 ELISA Kit; APOC2 protein ELISA Kit; APOC2_HUMAN ELISA Kit; ApoCII ELISA Kit; Apolipoprotein C II ELISA Kit; Apolipoprotein C II precursor ELISA Kit; Apolipoprotein C2 ELISA Kit; ApolipoproteinCII ELISA Kit; MGC75082 ELISA Kit; ProapoC-II ELISA Kit; Proapolipoprotein C-II ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.2 ng/mL-2000 ng/mL
  • 靈敏度：
    15.6 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human apo-C2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %98
    Range %90-102
    1:200Average %88
    Range %82-92
    1:400Average %100
    Range %90-104
    1:800Average %101
    Range %91-105
  • 回收率：
    The recovery of human apo-C2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9990-102
    EDTA plasma (n=4)9288-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    20000.088 0.093 0.091
    10000.215 0.221 0.218
    5000.415 0.424 0.420
    2500.693 0.682 0.688
    1251.095 1.077 1.086
    62.51.521 1.500 1.511
    31.21.668 1.598 1.633
    02.034 1.987 2.011
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase. Both proapolipoprotein C-II and apolipoprotein C-II can activate lipoprotein lipase. In normolipidemic individuals, it is mainly distributed in the HDL, whereas in hypertriglyceridemic individuals, predominantly found in the VLDL and LDL.
  • 基因功能參考文獻(xiàn)：
    1. Results from this exploratory analysis suggest that regulatory element methylation levels within the larger TOMM40-APOE-APOC2 gene region correlate with AD-related biomarkers and TOMM40 or APOE gene expression in AD. PMID: 29371683
    2. Triglyceride-raising variant alleles of the APOC2 encoding apo C-II, associated with clinical Cardiovascular endpoints. PMID: 28534127
    3. The results demonstrate the important role of both intra- and inter-subunit charge interactions in stabilizing apoC-II amyloid fibrils, a process that may be a key factor in determining the general ability of proteins to form amyloid fibrils. PMID: 28229588
    4. The results highlight the importance of charge-pair interactions within the apoC-II fibril core PMID: 26196342
    5. Conformational rearrangement of apoC-II at lipoprotein surfaces promotes interaction with LPL. PMID: 26026161
    6. Large deletion in APOC2 caused by Alu-Alu homologous recombination is associated with with apolipoprotein C-II deficiency. PMID: 25172036
    7. No APOC2 mutations were identified in a cohort of patients with diabetic lipemia. PMID: 25131724
    8. STAT1 bound on multienhancer 2 cooperates with RXRalpha located on apoCII promoter and upregulates apoCII expression only in macrophages. PMID: 22808166
    9. Mutations in GPIHBP1 are rare but the associated clinical phenotype of hypertriglyceridaemia is severe PMID: 22239554
    10. These results support a predictive change in the ratio of plasma ApoCIII to ApoCII in pregnancies complicated by severe preeclampsia. PMID: 21321243
    11. Substoichiometric concentrations of cyc[60-70] significantly delayed fibril formation by the fibrillogenic, linear peptides apoC-II[60-70] and apoC-II[56-76]. PMID: 22244853
    12. Activation of apoC-II fibrils by submicellar lipid (NBD-lyso-12-phosphocholine) is catalytic with release of monomer- and tetramer-bound lipid accompanying fibril elongation and growth. PMID: 21985034
    13. Physiological shear flow conditions and conditions experienced during apoC-II manufacturing exert significant effects on apoC-II conformation, leading to protein misfolding, aggregation, and amyloid fibril formation. PMID: 21476595
    14. Includes the observation of APOC4-APOC2 read-through transcription PMID: 8530039
    15. Our structural model for apoC-II fibrils suggests that apoC-II monomers fold and self-assemble to form a stable cross-beta-scaffold containing relatively unstructured connecting loops. PMID: 21146539
    16. Results describe the functional role of the secondary structure in the lipoprotein lipase-binding portion of apolipoprotein CII. PMID: 20042600
    17. Human apolipoprotein C-II (apoC-II) slowly forms amyloid fibers in lipid-free solutions at physiological pH and salt concentrations PMID: 11751863
    18. During amyloidosis under oxidizing conditions, cysteine-containing apolipoprotein C-II (apoC-II) derivatives form fibrils more rapidly and become extensively tangled compared to wild-type apoC-II. PMID: 12450397
    19. Three categories of global constraints, together with the local classical NMR constraints, define the 3D structure of the apoCII-SDS micelle complex and give important clues toward a possible mechanism for the activation of lipoprotein lipase by apoCII. PMID: 12590574
    20. regions of lipoprotein lipase that are responsive to activation by apoC-II PMID: 12682050
    21. Hydrolysis activated by APOC2 was faster compared with the LPL-mediated lipolysis of emulsion triolein. The binding density of APOC2 was less for small emulsion surfaces than for large ones. PMID: 12782148
    22. Different levels of secreted apoC-II had little effect on LDL and HDL protein degradation by HepG2 cells. Compared to controls, cells under-expressing apoC-II showed a 160% higher capacity to selectively take up HDL-CE. PMID: 15778093
    23. Results show that purified human HDL and recombinant apolipoprotein A-I lipid particles bind directly to amyloid beta and apolipoprotein C-II amyloid fibrils. PMID: 16432277
    24. No relationship was found between ApoCII polymorphism and coronary disease in the Chinese Han population. PMID: 16459141
    25. Decrease of LPL activity in the heart, along with the inhibitory effects of excess apolipoprotein C-II, may contribute to the hypertriglyceridemia observed in apolipoprotein c-ii transgenic mice. PMID: 17018885
    26. Taken together these data demonstrate an interaction between antichymotrypsin and apolipoprotein C-II that accelerates fibrillogenesis and indicates a specific role for accessory proteins in protein aggregation. PMID: 17174330
    27. These results suggested that T-->A substitution at position -190 in the apoC-II gene promoter only partly affected transcriptional activity of the apoC-II promoter, leading to decrease of apoC-II expression in quantity. PMID: 17222387
    28. The ozone oxidation product of cholesterol, 3beta-hydroxy-5-oxo-5,6-secocholestan-6-al, rapidly promotes human apolipoprotein (apo) C-II amyloid fibril formation in vitro. PMID: 17429947
    29. Both common and rare DNA variants of APOC2 gene were found in 10% patients with severe hypertriglyceridemia PMID: 17717288
    30. Phospholipid interaction induces molecular-level polymorphism in APOC2 amyloid fibrils via alternative assembly pathways. PMID: 18005990
    31. The concentration-dependent kinetics of apolipoprotein C-II amyloid fibril formation and correlated this with the final size distribution of the fibrils determined by sedimentation velocity experiments, is studied. PMID: 18206908
    32. lipids promote on-pathway intermediates of apoC-II fibril assembly and the accumulation of a discrete tetrameric intermediate depends on the molecular state of the lipid PMID: 18852267
    33. No significant differences were found between the acute hypertriglyceridaemic pancreatitis cases and controls with severe hypertriglyceridaemia in terms of LPL activity and mass, hepatic lipase activity, CII and CIII mass, or apo E polymorphisms. PMID: 19534808

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  • 相關(guān)疾?。?/div>
    Hyperlipoproteinemia 1B (HLPP1B)
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein C2 family
  • 組織特異性：
    Liver and intestine.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 609

    OMIM: 207750

    KEGG: hsa:344

    STRING: 9606.ENSP00000466775

    UniGene: Hs.75615