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  4. Human Aquaporin 4 Antibody,AQP-4 Ab ELISA Kit

Human Aquaporin 4 Antibody,AQP-4 Ab ELISA Kit

  • 中文名稱：
    人水通道蛋白4抗體(AQP-4 Ab)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13568h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人水通道蛋白4抗體(AQP-4 Ab)酶聯(lián)免疫試劑盒（CSB-E13568h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的AQP-4 Ab 含量。AQP - 4 Ab是一種靶點(diǎn)。AQP - 4即水通道蛋白4，其抗體（Ab）在神經(jīng)系統(tǒng)疾病尤其視神經(jīng)脊髓炎譜系疾病中意義重大。研究機(jī)制上，它可結(jié)合AQP - 4，介導(dǎo)補(bǔ)體激活、免疫細(xì)胞浸潤(rùn)等免疫病理過(guò)程，損傷神經(jīng)組織。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，適用于科研場(chǎng)景中疾病模型構(gòu)建、抗體表達(dá)水平研究、藥物干預(yù)效果評(píng)估等實(shí)驗(yàn)需求；為探索 AQP - 4 抗體在神經(jīng)免疫疾病中的分子機(jī)制、篩選潛在治療靶點(diǎn)等基礎(chǔ)研究提供可靠工具，檢測(cè)數(shù)據(jù)可服務(wù)于細(xì)胞實(shí)驗(yàn)、動(dòng)物模型研究及生物標(biāo)志物分析等方向。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫：
    AQP-4 Ab
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 82-95  
    1:2 Average % 94  
    Range % 84-105  
    1:4 Average % 97  
    Range % 92-101  
    1:8 Average % 91  
    Range % 82-97  
  • 回收率：
    The recovery of human AQP-4 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 82-197  
    EDTA plasma (n=4) 95 84-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    100 2.615 2.575 2.595 2.456  
    50 1.988 2.103 2.046 1.907  
    25 1.299 1.312 1.306 1.167  
    12.5 0.672 0.675 0.674 0.535  
    6.25 0.407 0.392 0.400 0.261  
    3.12 0.294 0.278 0.286 0.147  
    1.56 0.225 0.214 0.220 0.081  
    0 0.142 0.135 0.139 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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