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  4. Human Aspartate aminotransferase,AST ELISA Kit

Human Aspartate aminotransferase,AST ELISA Kit

  • 中文名稱：
    人天門冬氨酸氨基轉(zhuǎn)移酶(AST)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09603h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人天門冬氨酸氨基轉(zhuǎn)移酶(AST)酶聯(lián)免疫試劑盒（CSB-E09603h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GOT1含量。GOT1即天冬氨酸轉(zhuǎn)氨酶 1，在細(xì)胞代謝尤其是氨基酸代謝、三羧酸循環(huán)中起關(guān)鍵作用。研究發(fā)現(xiàn)其在腫瘤發(fā)生發(fā)展中機制復(fù)雜，它參與維持腫瘤細(xì)胞增殖所需代謝物供應(yīng)，還與腫瘤細(xì)胞的氧化還原穩(wěn)態(tài)相關(guān)，是潛在的腫瘤治療靶點。試劑盒檢測范圍為0.312 mU/ml- 20 mU/ml，靈敏度為0.078 mU/ml。在基礎(chǔ)醫(yī)學(xué)研究中廣泛用于評估肝細(xì)胞功能狀態(tài)、藥物毒性作用或心血管疾病模型的病理進程；特別適用于體外實驗中監(jiān)測細(xì)胞或組織樣本的 AST 釋放量，例如在肝損傷動物模型研究中評估組織勻漿酶活性變化，或通過血清/血漿樣本分析代謝性疾病進程中細(xì)胞膜的完整性。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AATC_HUMAN ELISA Kit; Aspartate aminotransferase 1 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase cytoplasmic ELISA Kit; Aspartate aminotransferase cytosolic ELISA Kit; ASTQTL1 ELISA Kit; cAspAT ELISA Kit; cCAT ELISA Kit; cysteine aminotransferase; cytoplasmic ELISA Kit; cysteine transaminase; cytoplasmic ELISA Kit; cytoplasmic ELISA Kit; ec 2.6.1.1 ELISA Kit; GIG 18 ELISA Kit; GIG18 ELISA Kit; Glutamate oxaloacetate transaminase 1 ELISA Kit; Glutamate oxaloacetate transaminase soluble ELISA Kit; Glutamic oxaloacetic transaminase 1 soluble ELISA Kit; GOT 1 ELISA Kit; GOT1 ELISA Kit; Growth inhibiting protein 18 ELISA Kit; SGOT ELISA Kit; Transaminase A ELISA Kit
  • 縮寫：
    GOT1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 mU/ml - 20 mU/ml
  • 靈敏度：
    0.078 mU/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(mU/ml)

    2.283

    2.469

    2.072

    2.060

    2.283

    2.346

    SD

    0.044

    0.045

    0.038

    0.038

    0.044

    0.047

    CV(%)

    6.607

    6.475

    6.022

    6.041

    6.607

    6.953

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-88

    1:2

    Average %

    93

    Range %

    88-94

    1:4

    Average %

    95

    Range %

    90-99

    1:8

    Average %

    103

    Range %

    97-110

  • 回收率：

    The recovery of human AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    90

    85-94

    EDTA plasma(n=4)

    95

    88-99

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    mU/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.368

    2.401

    2.384

    2.251

    10

    1.439

    1.442

    1.440

    1.307

    5

    1.015

    1.006

    1.011

    0.878

    2.5

    0.665

    0.642

    0.654

    0.521

    1.25

    0.441

    0.435

    0.438

    0.305

    0.625

    0.347

    0.351

    0.349

    0.216

    0.312

    0.201

    0.204

    0.203

    0.070

    0

    0.135

    0.132

    0.133

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

I would like to know if the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.

A:
Thanks for your inquiry.The kit is developed for the human matrix to quantitatively detect the content of the corresponding protein.
Some components of the kit contain BSA,there is no HIV / AIDS / HCV / HBV / infective material.
All kits are for scientific research use, there is no infectious component and can be used in accordance with conventional biosecurity rules of operation.

靶點詳情

  • 最新研究進展：
    GOT1，即天冬氨酸轉(zhuǎn)氨酶1，是一種酶，參與氨基酸代謝。最新的研究表明，GOT1在某些癌癥的發(fā)生和發(fā)展中發(fā)揮著重要作用。例如，研究表明，GOT1在肝癌和結(jié)腸癌中的表達水平升高，并且與腫瘤的分化程度和惡性程度密切相關(guān)。此外，一些研究表明，抑制GOT1的活性可能是一種治療肝癌和結(jié)腸癌的新策略
  • 功能：
    Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
  • 基因功能參考文獻：
    1. high transaminase elevations do not predict severe complications or reflect remnant ischemic area. PMID: 29553104
    2. Missense variant (p.Gln208Glu, rs374966349) in glutamate oxaloacetate transaminase 1 (GOT1) was found, as a putative causal variant predisposing to Familial Macro-aspartate aminotransferase . PMID: 28716744
    3. HIF-2a regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway and GOT1 expression in human pancreatic ductal adenocarcinoma. PMID: 28544376
    4. ALT is a more suitable index than AST for developing a regression model. PMID: 28628604
    5. High AST1 expression is associated with high mortality in hemodialysis patients. PMID: 26333544
    6. GOT1 plays an important role in energy metabolism and ROS balance in chronic acidosis stress in pancreatic tumor cells. PMID: 28455244
    7. Early abnormal liver enzyme levels of aspartate aminotransferase and alanine aminotransferase may increase the prevalence of human cytomegalovirus antigenemia after hematopoietic stem cell transplantation. PMID: 28415934
    8. Elevated preoperative aspartate aminotransferase-to-platelet ratio index may be independently associated with poor overall survival and disease-free survival in hepatocellular carcinoma patients following curative resection PMID: 28351330
    9. Elevated levels of ALT are Associated with Cardiovascular disease. PMID: 27872510
    10. Elevated aspartate aminotransferase level was associated with hepatocellular carcinoma. PMID: 26881519
    11. The serum hepcidin, ferritin, alanine transaminase, aspartate transaminase, gamma-glutamyltransferase and bilirubin were examined in two independent Chinese cohorts consisted of 3455 individuals. PMID: 26290281
    12. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GOT1 rises due to up-regulation of hepatic expression of GOT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
    13. Human GOT1 was expressed in E coli, purified by affinity chromatography and preliminary crystallographic studies were done. PMID: 26003525
    14. We propose the GOT1 expression status as a simple and reliable prognostic biomarker in pancreatic ductal adenocarcinoma PMID: 25595905
    15. Significantly higher GOT levels were found in the 1-20 year age group of type 1 PMID: 24849491
    16. The aim of this study was to establish the reference intervals (RIs) of total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate transaminase (AST), and creatinine (CREA) for apparently healthy elderly (Han ethnicity) in Shuyang, China. PMID: 25272068
    17. APRI and AST/ALT are independent predictors for significant fibrosis in chronic hepatitis C patients. PMID: 22352690
    18. This study highlights the necessity of intensive follow-up for those with elevated AST and ALT levels and comorbid alcohol use disorder for preventing excessive natural deaths. PMID: 22242166
    19. we discovered a rare in-frame deletion in GOT1 gene, which inactivates cAST enzyme in the Old Order Amish. PMID: 21900944
    20. Data indicate that the percentages of CD4(+)CXCR5(+) TFH in IA patients were positively correlated with AST. PMID: 21750724
    21. liver enzymes, including AST, were significantly associated with IR according to direct clamp assessment, which were independent of the traditional risk factors in diabetic patients PMID: 20972737
    22. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
    23. mildly elevated in severe acute respiratory syndrome patients during second week after onset of fever. PMID: 15306699
    24. saliva AST and ALP may have roles in development of periodontal diseases PMID: 16681433
    25. PTLP activity is positively associated with serum ALT1, GOT1 and GOT2 in type 2 diabetes mellitus and metabolic syndrome. PMID: 17877759
    26. Abnormal serum aminotransferase values are uncommon in severely obese children in France. PMID: 17907318
    27. Data revealed higher aspartate aminotransferase for women with a family history of diabetes, when adjusted for age and BMI. PMID: 18242760
    28. Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
    29. Neither ALT nor GGT concentrations were correlated with ALP concentration, but AST concentration was moderately correlated with ALP concentration. PMID: 19012630
    30. Results suggest that alanine and aspartate aminotransferase levels and AST/ALT ratio do not seem to be reliable predictors for non-alcoholic steatohepatitis. PMID: 19370784
    31. the management of patients with pyelonephritis should take into account that moderate and self-limited abnormalities in aminotransferase levels are frequent during the acute phase of the disease PMID: 19393479

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4432

    OMIM: 138180

    KEGG: hsa:2805

    STRING: 9606.ENSP00000359539

    UniGene: Hs.500756