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  4. Human Azurocidin/Heparin Binding Protein(AZU/HBP) ELISA Kit

Human Azurocidin/Heparin Binding Protein(AZU/HBP) ELISA Kit

  • 中文名稱：
    人天青殺素/肝素結(jié)合蛋白(AZU/HBP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09698h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人天青殺素/肝素結(jié)合蛋白(AZU/HBP)酶聯(lián)免疫試劑盒（CSB-E09698h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織勻漿、組織培養(yǎng)上清液樣本中的AZU1含量。AZU1，也稱為肝素結(jié)合蛋白（HBP）和陽離子抗菌蛋白CAP37，是一種由中性粒細(xì)胞顆粒釋放的抗菌和趨化性糖蛋白，屬于肽酶S1家族和彈性酶亞家族。它主要對革蘭氏陰性細(xì)菌具有細(xì)胞毒性，可能參與炎癥過程中單核細(xì)胞的募集。研究顯示，AZU1在急性早幼粒細(xì)胞白血?。ˋPL）中表達(dá)升高，可能與白血病細(xì)胞的分化和治療反應(yīng)有關(guān)。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，支持研究者定量分析AZU/HBP在炎癥反應(yīng)、免疫調(diào)控或感染模型中的動態(tài)變化，為探索其作為炎癥相關(guān)生物標(biāo)志物的功能機制提供可靠工具，廣泛應(yīng)用于免疫學(xué)、細(xì)胞生物學(xué)及疾病機制等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, tissue homogenates, cell culture supernates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human AZU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 80-91  
    1:2 Average % 95  
    Range % 90-100  
    1:4 Average % 98  
    Range % 92-107  
    1:8 Average % 92  
    Range % 82-98  
  • 回收率：
    The recovery of human AZU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 80-98  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 2.898 2.682 2.790 2.686  
    250 2.531 2.486 2.509 2.405  
    125 2.147 2.045 2.096 1.992  
    62.5 1.602 1.501 1.552 1.448  
    31.2 0.887 0.872 0.880 0.776  
    15.6 0.504 0.493 0.499 0.395  
    7.8 0.278 0.256 0.267 0.163  
    0 0.107 0.100 0.104 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

ELISA試劑盒說明書上說標(biāo)準(zhǔn)品重建后需在4小時內(nèi)使用，但相關(guān)產(chǎn)品上標(biāo)注的Recombinant Human Azurocidin可在一周內(nèi)使用。我想知道4小時后這個標(biāo)準(zhǔn)是否還能用？

A:
通常，我們不會把它寫在說明書里。溶解后的標(biāo)準(zhǔn)品不含任何污染物，可保存一周。但如果用不同的梯度稀釋溶解的標(biāo)準(zhǔn)品，則應(yīng)在4小時內(nèi)用完。

靶點詳情

  • 功能：
    This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
  • 亞細(xì)胞定位：
    Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Peptidase S1 family, Elastase subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 913

    OMIM: 162815

    KEGG: hsa:566

    STRING: 9606.ENSP00000233997

    UniGene: Hs.72885