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  4. Human Bleomycin hydrolase(BLMH) ELISA kit

Human Bleomycin hydrolase(BLMH) ELISA kit

  • 中文名稱：
    人博來霉素水解酶(BLMH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL002716HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人博來霉素水解酶(BLMH)酶聯(lián)免疫試劑盒（CSB-EL002716HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的BLMH含量。BLMH（博萊霉素水解酶）是一種在進化中高度保守的胞質(zhì)半胱氨酸肽酶，主要功能是代謝失活抗腫瘤藥物博萊霉素（BLM），保護細胞免受其毒性作用。其研究機制涉及對BLM的羧酰胺鍵進行水解，維持細胞平衡和防御有害物質(zhì)。試劑盒檢測范圍為28 pg/mL-1800 pg/mL，適用于體外培養(yǎng)細胞模型、動物組織樣本或生物體液中的BLMH蛋白定量分析，為探索其在細胞應激調(diào)控、藥物代謝機制研究、疾病相關(guān)分子標志物篩選等科研方向提供精準檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BH ELISA Kit; Bleomycin hydrolase ELISA Kit; BLM hydrolase ELISA Kit; BLMH ELISA Kit; BLMH_HUMAN ELISA Kit; BMH ELISA Kit; EC 3.4.22.40 ELISA Kit
  • 縮寫：
    BLMH
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    28 pg/mL-1800 pg/mL
  • 靈敏度：
    7 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BLMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %110
    Range %107-113
    1:2Average %89
    Range %84-93
    1:4Average %85
    Range %81-89
    1:8Average %86
    Range %83-89
  • 回收率：
    The recovery of human BLMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9491-99
    EDTA plasma (n=4)105101-109
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    18002.364 2.329 2.347 2.225
    9001.526 1.568 1.547 1.425
    4500.961 0.975 0.968 0.846
    2250.592 0.578 0.585 0.463
    112.50.383 0.372 0.378 0.256
    560.298 0.271 0.285 0.163
    280.197 0.189 0.193 0.071
    00.121 0.123 0.122
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
  • 基因功能參考文獻：
    1. the BLMH gene single nucleotide polymorphism A1450G (rs1050565) influences BLMH activity and late pulmonary toxicity. PMID: 27327270
    2. Ubc9 plays different roles of action in antitumor agents in chemotherapy. The process requires bleomycin hydrolase and poly(ADP-ribose) polymerase-1. The results are beneficial to deeply understanding of Ubc9 functions and for precise prediction of chemotherapy outcomes in tumors. PMID: 27878232
    3. Bleomycin hydrolase downregulation in lesional skin of adult atopic dermatitis patients is independent of filaggrin gene mutations PMID: 25240784
    4. This study findings suggest that Blmh interacts with diverse cellular processes from energy metabolism and anti-oxidative defenses to cell cycle, cytoskeleton dynamics, and synaptic plasticity essential for normal brain homeostasis. PMID: 24496069
    5. We also detected significant association between XRCC1, XRCC3, and BLHX polymorphisms and a high frequency of chromosomal damage PMID: 24615029
    6. The caspase-dependent cleavage of BLH was confirmed by cleavage of partly-purified human bleomycin hydrolase with caspase-3. PMID: 23708668
    7. BH may play an important role during the late stage of epidermal differentiation. PMID: 22037625
    8. present study suggests that our new method can detect novel genes of interest and that BLMH is a suppressor gene in HCC PMID: 21943823
    9. This first report on BLMH carrier status in Tunisia shows o association between carrying the BLMH-G genotype and Alzheimer's disease in epsilon4 negative or positive subjects. PMID: 20198498
    10. Cysteine proteases bleomycin hydrolase and cathepsin Z mediate N-terminal proteolysis and toxicity of mutant huntingtin. PMID: 21310951
    11. BH activity and expression were markedly decreased in AD lesional skin, suggesting a defect of the filaggrin degradation pathway in AD. PMID: 21190945
    12. Polymorphism is associated with neurodegenerative diseases, notably Alzheimer disease. PMID: 12604387
    13. Significant effect of BLHX variant alleles (A/G, G/G) on the chromosome damage induced by bleomycin. PMID: 18082847
    14. The homozygous variant G/G of BLMH gene SNP A1450G is associated with reduced survival and higher prevalence of early relapses in TC patients treated with bleomycin-containing chemotherapy. PMID: 18398146

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  • 亞細胞定位：
    Cytoplasm. Cytoplasmic granule.
  • 蛋白家族：
    Peptidase C1 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1059

    OMIM: 602403

    KEGG: hsa:642

    STRING: 9606.ENSP00000261714

    UniGene: Hs.371914