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  4. Human Bone morphogenetic protein 8B(BMP8B) ELISA kit

Human Bone morphogenetic protein 8B(BMP8B) ELISA kit

  • 中文名稱：
    人骨形態(tài)發(fā)生蛋白8B(BMP8B)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL002746HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人骨形態(tài)發(fā)生蛋白8B(BMP8B)酶聯(lián)免疫試劑盒（CSB-EL002746HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BMP8B含量。BMP8B屬于骨形態(tài)發(fā)生蛋白家族成員。在背景上，它參與多種生理進(jìn)程。研究機制方面，其能調(diào)控細(xì)胞的增殖、分化和凋亡等，在骨代謝、生殖系統(tǒng)等方面發(fā)揮作用，深入研究它對理解相關(guān)生理病理過程有重要意義。試劑盒檢測范圍為9.37 pg/mL-600 pg/mL，該產(chǎn)品適用于體外研究BMP8B在骨代謝調(diào)控機制、生殖系統(tǒng)功能分析或特定病理模型（如骨發(fā)育異常、組織纖維化等）中的動態(tài)表達(dá)特征，為探索其分子作用機制提供可靠工具。實驗操作流程簡便，兼容常規(guī)實驗室設(shè)備，支持多樣本類型分析，尤其適用于需要高靈敏度檢測的細(xì)胞或動物模型研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BMP-8 ELISA Kit; BMP-8B ELISA Kit; BMP8 ELISA Kit; Bmp8b ELISA Kit; BMP8B_HUMAN ELISA Kit; bone morphogenetic protein 8 (osteogenic protein 2) ELISA Kit; Bone morphogenetic protein 8 ELISA Kit; Bone morphogenetic protein 8B ELISA Kit; dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)) ELISA Kit; OP-2 ELISA Kit; OP2 ELISA Kit; Osteogenic protein 2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    9.37 pg/mL-600 pg/mL
  • 靈敏度：
    2.34 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BMP8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 94  
    Range % 90-98  
    1:10 Average % 88  
    Range % 82-94  
    1:20 Average % 103  
    Range % 99-108  
    1:40 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of human BMP8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 98 94-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    600 2.056 2.205 2.131 2.008  
    300 1.607 1.724 1.666 1.543  
    150 1.102 1.075 1.089 0.966  
    75 0.645 0.603 0.624 0.501  
    37.5 0.394 0.373 0.384 0.261  
    18.75 0.291 0.302 0.297 0.174  
    9.37 0.187 0.201 0.194 0.071  
    0 0.120 0.125 0.123    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
  • 基因功能參考文獻(xiàn)：
    1. Acetylated H3K9 and H4K16 levels at BMP8B were increased in gastric cancer (GC) compared to nontumors (P < 0.05). However, reduced levels of acetylated H3K9 and H4K16 were associated with poorly differentiated GC (P < 0.05). PMID: 27748538
    2. Study found that BMP8B was downregulated in pancreatic cancer tissues and BMP8B mediates the survival of pancreatic cancer cells and regulates the progression of pancreatic cancer. PMID: 25176058
    3. Five novel SNPs within BMP8B gene.Genetic variants in BMP8B gene are associated with growth traits in Chinese native cattle. PMID: 24076131
    4. Epigenetic silencing of the bone morphogenetic protein 2 gene through methylation is associated with gastric carcinomas PMID: 16314833
    5. Reports on the mouse homolog of this gene. PMID: 9070944

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1075

    OMIM: 602284

    KEGG: hsa:656

    STRING: 9606.ENSP00000361915

    UniGene: Hs.734367